Anti-FOXO1A (phospho S322 + S325) 抗体 (ab60945)
Key features and details
- Rabbit polyclonal to FOXO1A (phospho S322 + S325)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-FOXO1A (phospho S322 + S325) antibody
FOXO1A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXO1A (phospho S322 + S325) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human FOXO1A (phospho S322 + S325). Synthetic phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 322 and serine 325 (N-A-SP-T-I-SP-G-R).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab60945 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab60945の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa). |
ターゲット情報
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機能
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. -
組織特異性
Ubiquitous. -
関連疾患
Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
翻訳後修飾
Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 56458 Mouse
- Entrez Gene: 84482 Rat
- SwissProt: Q9R1E0 Mouse
- SwissProt: G3V7R4 Rat
- Unigene: 29891 Mouse
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別名
- FKH 1 antibody
- FKH1 antibody
- FKHR antibody
see all
画像
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Western blot - Anti-FOXO1A (phospho S322 + S325) antibody (ab60945)All lanes : Anti-FOXO1A (phospho S322 + S325) antibody (ab60945) at 1/500 dilution
Lane 1 : NIH 3T3 cell extracts treated with Wortmannin (40nM, 24hours)
Lane 2 : NIH 3T3 cell extracts treated with Wortmannin (40nM, 24hours) with immunising peptide
Predicted band size: 70 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab60945 は論文での使用が確認できていません。