Anti-FOXO1A 抗体 (ab70382)
Key features and details
- Rabbit polyclonal to FOXO1A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXO1A antibody
FOXO1A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXO1A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig
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免疫原
Synthetic peptide within Human FOXO1A aa 300 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q12778 -
ポジティブ･コントロール
- WB: HepG2, HEK-293T and RKO whole cell lysate. IP: HepG2 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70382の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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| IP | (1) |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. -
組織特異性
Ubiquitous. -
関連疾患
Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
翻訳後修飾
Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 506618 Cow
- Entrez Gene: 2308 Human
- Entrez Gene: 56458 Mouse
- Entrez Gene: 397077 Pig
- Entrez Gene: 84482 Rat
- Omim: 136533 Human
- SwissProt: Q12778 Human
- SwissProt: Q9R1E0 Mouse
see all -
別名
- FKH 1 antibody
- FKH1 antibody
- FKHR antibody
see all
画像
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Western blot - Anti-FOXO1A antibody (ab70382)All lanes : Anti-FOXO1A antibody (ab70382) at 0.1 µg/ml
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : RKO (Human colon carcinoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 70 kDa
Exposure time: 3 minutesLysates prepared using NTEN lysis buffer.
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Immunoprecipitation - Anti-FOXO1A antibody (ab70382)Detection of human FOXO1A by western blot of immunoprecipitates.
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HepG2 (Human liver hepatocellular carcinoma cell line) cells prepared using NETN lysis buffer.
Antibodies: ab70382 used for IP at 6 µg per reaction. FOXO1A was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated ab70382 was used at 0.1 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab70382 は 21 報の論文で使用されています。
- Zhao HY et al. L-carnitine treatment attenuates renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction. Korean J Intern Med 36:S180-S195 (2021). PubMed: 32942841
- Jeon HH et al. Clinical application of a FOXO1 inhibitor improves connective tissue healing in a diabetic minipig model. Am J Transl Res 13:781-791 (2021). PubMed: 33594326
- Fangma Y et al. Hydroxysafflor Yellow A and Anhydrosafflor Yellow B Protect Against Cerebral Ischemia/Reperfusion Injury by Attenuating Oxidative Stress and Apoptosis via the Silent Information Regulator 1 Signaling Pathway. Front Pharmacol 12:739864 (2021). PubMed: 34658877
- Wang RK et al. MicroRNA-124-5p delays the progression of cerebral aneurysm by regulating FoxO1. Exp Ther Med 22:1172 (2021). PubMed: 34504617
- Zhu L et al. Protective effect of hydrogen sulfide on endothelial cells through Sirt1-FoxO1-mediated autophagy. Ann Transl Med 8:1586 (2020). PubMed: 33437785