Anti-FOXM1 抗体 (ab245309)
Key features and details
- Rabbit polyclonal to FOXM1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXM1 antibody
FOXM1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXM1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human FOXM1 aa 713-763. The exact sequence is proprietary. NP_973731.1
Database link: Q08050-3 -
ポジティブ･コントロール
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245309 was affinity purified using an epitope specific to FOXM1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245309の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 89 kDa.
|
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| IP |
Use at 2-10 µg/mg of lysate.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 89 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Transcriptional activatory factor. May play a role in the control of cell proliferation. -
組織特異性
Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
発生段階
Embryonic expression pattern: liver, lung, intestine, kidney, urinary tract; adult expression pattern: intestine, colon, testis and thymus. -
ドメイン
Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4. -
翻訳後修飾
Phosphorylated in M (mitotic) phase. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2305 Human
- Omim: 602341 Human
- SwissProt: Q08050 Human
- Unigene: 239 Human
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別名
- FKHL16 antibody
- Forkhead box M1 antibody
- Forkhead box protein M1 antibody
see all
画像
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Western blot - Anti-FOXM1 antibody (ab245309)All lanes : Anti-FOXM1 antibody (ab245309) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 89 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-FOXM1 antibody (ab245309)FOXM1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245309 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab245309 at 1 µg/ml.
Lane 1: ab245309 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared using NETN lysis buffer.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab245309 は 4 報の論文で使用されています。
- Meng F et al. Knockdown of TRIM44 inhibits the progression of ovarian cancer and is related to the FOXM1-EZH2 signaling pathway. Transl Cancer Res 11:414-425 (2022). PubMed: 35281418
- Zhu Y et al. Cancer-Secreted Exosomal MiR-620 Inhibits ESCC Aerobic Glycolysis via FOXM1/HER2 Pathway and Promotes Metastasis. Front Oncol 12:756109 (2022). PubMed: 35651785
- Huang X et al. PRRX1/FOXM1 reduces gemcitabin-induced cytotoxicity by regulating autophagy in bladder cancer. Transl Androl Urol 11:1116-1129 (2022). PubMed: 36092834
- Wang G et al. LINC01410/miR-3619-5p/FOXM1 Feedback Loop Regulates Papillary Thyroid Carcinoma Cell Proliferation and Apoptosis. Cancer Biother Radiopharm 34:572-580 (2019). PubMed: 31644316