Anti-FOXL2 抗体 [262C1a] (ab58622)
![Western blot - Anti-FOXL2 antibody [262C1a] (ab58622)](https://www.abcam.co.jp/ps/products/58/ab58622/Images/ab58622-72531-anti-foxl2-antibody-262c1a-western-blot.gif "Western blot - Anti-FOXL2 antibody [262C1a] (ab58622)")
Key features and details
- Mouse monoclonal [262C1a] to FOXL2
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
-
製品名
Anti-FOXL2 antibody [262C1a]
FOXL2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [262C1a] to FOXL2 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment: NSIRHNLSLN ECFIKVPREG GGERKGNYWT LDPACEDMFE KGNYRRRRRM KRPFRPPPAH FQPGKGLFGA GGAAGGCGVA GAGADGYGYL APPKYLQSGF LN, corresponding to amino acids 100-201 of Human FOXL2
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
262C1a -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58622の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. |
ターゲット情報
-
機能
Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. -
組織特異性
In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary. -
関連疾患
Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
翻訳後修飾
Sumoylated by SUMO1; sumoylation is required for transcriptional repression activity. -
細胞内局在
Nucleus. - Information by UniProt
-
別名
- Blepharophimosis antibody
- Blepharophimosis epicanthus inversus and ptosis 1 antibody
- Blepharophimosis epicanthus inversus and ptosis antibody
see all
画像
-
Western blot - Anti-FOXL2 antibody [262C1a] (ab58622)Anti-FOXL2 antibody [262C1a] (ab58622) + immunogen (recombinant fragment)
Predicted band size: 42 kDa
The low MW of the band is due to the fact that the immunising recombinant fragment, rather than the full lenght protein, was used as positive control.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab58622 は論文での使用が確認できていません。
