Anti-FOXI1 抗体 (ab153935)
Key features and details
- Rabbit polyclonal to FOXI1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXI1 antibody
FOXI1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXI1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Zebrafish
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免疫原
Recombinant fragment, corresponding to a region within amino acids 76-262 of Human FOXI1 (Uniprot ID: Q12951).
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ポジティブ･コントロール
- Human Huh7 xenograft tissue; HeLa cells; A549, HeLa, HepG2 and HCT116 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153935の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
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| ICC/IF | (1) |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Transcriptional activator required for the development of normal hearing, sense of balance and kidney function. Required for the expression of SLC26A4/PDS, JAG1 and COCH in a subset of epithelial cells and the development of the endolymphatic system in the inner ear. Also required for the expression of SLC4A1/AE1, SLC4A9/AE4, ATP6V1B1 and the differentiation of intercalated cells in the epithelium of distal renal tubules. -
組織特異性
Expressed in kidney. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2299 Human
- Entrez Gene: 14233 Mouse
- Entrez Gene: 287185 Rat
- Entrez Gene: 353313 Zebrafish
- Omim: 601093 Human
- SwissProt: Q12951 Human
- SwissProt: Q922I5 Mouse
- Unigene: 87236 Human
see all -
別名
- FKH10 antibody
- FKHL10 antibody
- Forkhead (Drosophila) like 10 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXI1 antibody (ab153935)Immunohistochemical analysis of paraffin-embedded Human Huh7 xenograft tissue, labeling FOXI1 with ab153935 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-FOXI1 antibody (ab153935)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling FOXI1 with ab153935 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342. -
Western blot - Anti-FOXI1 antibody (ab153935)Anti-FOXI1 antibody (ab153935) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 31 kDa
12% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab153935 は 1 報の論文で使用されています。
- Lei L et al. CFTR-rich ionocytes mediate chloride absorption across airway epithelia. J Clin Invest 133:N/A (2023). PubMed: 37581935