AB3394

Anti-FOXG1 抗体

Name: Anti-FOXG1 抗体 (ab3394) | Abcam
Brand: Abcam Limited
SKU: AB3394
Price: 76000 JPY
Availability: InStock
Rating: 1 (1 reviews)

Anti-FOXG1 antibody

(1 Review)

(5 Publications)

Goat Polyclonal FOXG1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human FOXG1 aa 450 to C-terminus.

別名を表示する

FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, FOXG1, Forkhead box protein G1, Brain factor 1, Brain factor 2, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, Forkhead-related protein FKHL1, Forkhead-related protein FKHL2, Forkhead-related protein FKHL3, BF-1, BF1, BF-2, BF2, hBF-2, HFK1, HFK2, HFK3

3 Images

Supplier Data

Western blot - Anti-FOXG1 antibody (AB3394)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-FOXG1 antibody (ab3394) at 0.5 µg/mL

Lane 1:

Human Cerebellum tissue lysate at 35 µg

Lane 2:

Cerebral Cortex tissue lysate at 35 µg

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-FOXG1 antibody (AB3394)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-FOXG1 antibody (ab3394) at 1 µg/mL

All lanes:

U251 cell lysate at 35 µg

Predicted band size: 54 kDa

false

Flow Cytometry - Anti-FOXG1 antibody (AB3394)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-FOXG1 antibody (AB3394)

Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, Flow Cyt

applications

免疫原

Synthetic Peptide within Human FOXG1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P55316

特異性

This antibody will recognise both human FOXG1A and FOXG1B, which are almost completely identical.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. The additonal unknown band at 70kDa was successfully blocked with the immunising peptide.</p>" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Constituents: Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FOXG1 also known as Brain Factor 1 (BF-1) is a transcription factor with a molecular weight of approximately 52 kDa. This protein plays an important role in the modulation of gene expression. FOXG1 is found mainly in the developing brain and other tissues of the central nervous system. In adult brains its expression levels significantly decrease although some residual expression persists in areas such as the hippocampus.

Biological function summary

FOXG1 regulates processes of neural development and brain function. It is not known to be part of a protein complex but FOXG1 binds to DNA influencing the transcriptional activity of genes involved in neurogenesis. The protein contributes to the development of structures such as the telencephalon and regulates transitions between different stages of neuronal cell development. Its role extends to maintaining the balance between proliferation and differentiation of neural progenitor cells.

Pathways

FOXG1 participates in the Notch and Wnt signaling pathways. These pathways are critical for cell fate determination and embryonic development. FOXG1 interacts with proteins like those in the Notch pathway to maintain neural progenitors in a proliferative state. In the Wnt signaling pathway FOXG1 helps in modulating the signaling that controls brain patterning working in concert with other transcription factors and signaling molecules.

FOXG1 mutations can lead to congenital conditions such as FOXG1 syndrome and Rett syndrome. In such disorders abnormalities in FOXG1 disrupt normal neural development leading to cognitive and developmental impairments. The involvement of FOXG1 with the MeCP2 protein in Rett syndrome highlights its importance in maintaining normal neurological function. Understanding FOXG1's interactions and functions provides insights into potential therapeutic approaches for these disorders.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.

See full target information FOXG1

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 115:2565-2577 PubMed38932521

2024

FoxG1/BNIP3 axis promotes mitophagy and blunts cisplatin resistance in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Baolong Pan,Yan Li,Huiyun Han,Lu Zhang,Xuemei Hu,Yanyu Pan,Zhuohui Peng

CNS neuroscience & therapeutics 29:1785-1804 PubMed36880283

2023

Transplantation of glutamatergic neuronal precursor cells in the paraventricular thalamus and claustrum facilitates awakening with recovery of consciousness.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Zhao,Naili Wei,Tianwen Li,Kezhu Chen,Wenqiang Cui,Zhifu Wang,Fan Wang,Yuanxiang Lin,Jianhong Zhu

Molecular psychiatry 28:497-514 PubMed35318461

2022

Differential vulnerability of adult neurogenic niches to dosage of the neurodevelopmental-disorder linked gene Foxg1.

Applications

Unspecified application

Species

Unspecified reactive species

Iris Schäffner,Marie-Theres Wittmann,Tanja Vogel,D Chichung Lie

Molecular endocrinology (Baltimore, Md.) 27:1113-27 PubMed23660594

2013

Transcriptional repression of AIB1 by FoxG1 leads to apoptosis in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan V Li,Christopher D Chien,Jason P Garee,Jianming Xu,Anton Wellstein,Anna T Riegel

Nature cell biology 9:531-40 PubMed17435750

2007

The neural progenitor-specifying activity of FoxG1 is antagonistically regulated by CKI and FGF.

Applications

ICC/IF

Species

Mouse

Tarik Regad,Martin Roth,Nicholas Bredenkamp,Nicola Illing,Nancy Papalopulu

View all publications

style

left-column

style

left-column

style

right-column