Anti-FOXF1 抗体 [EPR7971] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-FOXF1 antibody [EPR7971] - BSA and Azide free (AB240153)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FOXF1 with purified ab168383 at 1 : 250 dilution (2.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti foxf1 antibody epr7971 immunocytochemistry hela human)

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-FOXF1 antibody [EPR7971] - BSA and Azide free (AB240153)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FOXF1 with purified ab168383 at 1/70 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168383)

• WB

Lab

Western blot - Anti-FOXF1 antibody [EPR7971] - BSA and Azide free (AB240153)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168383).

This antibody detects high background

All lanes:

Western blot - Anti-FOXF1 antibody [EPR7971] (<a href='/products/primary-antibodies/foxf1-antibody-epr7971-ab168383'>ab168383</a>) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Human lung lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

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• WB

Lab

Western blot - Anti-FOXF1 antibody [EPR7971] - BSA and Azide free (AB240153)

We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background. This data was developed using the same antibody clone in a different buffer formulation (ab168383).

All lanes:

Western blot - Anti-FOXF1 antibody [EPR7971] (<a href='/products/primary-antibodies/foxf1-antibody-epr7971-ab168383'>ab168383</a>) at 1/1000 dilution

Lane 1:

Frozen HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Fresh HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

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Exposure time: 60s