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Anti-FOXD3 抗体 (ab67758)

Reviews (2)Q&A (1)References (6)

Key features and details

  • Rabbit polyclonal to FOXD3
  • Suitable for: ChIP, WB
  • Reacts with: Chicken
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-FOXD3 antibody
    FOXD3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to FOXD3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ChIP, WBmore details

  • 種交差性

    交差種: Chicken
    交差が予測される動物種: Mouse, Human

  • 免疫原

    Synthetic peptide derived from N terminal domain of chicken FOXD3 protein

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab67758の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ChIP (1)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
ChIP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.

  • 組織特異性

    Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.

  • 関連疾患

    Defects in FOXD3 are associated with susceptibility to autoimmune disease type 1 (AIS1) [MIM:607836]. AIS1 is a disorder characterized by the association of vitiligo with autoimmune thyroiditis (Hashimoto thyroiditis).

  • 配列類似性

    Contains 1 fork-head DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AIS1 antibody
    • forkhead box D3 antibody
    • Forkhead box protein D3 antibody
    • foxd3 antibody
    • FOXD3_HUMAN antibody
    • Genesis antibody
    • HFH2 antibody
    • HNF3/FH transcription factor genesis antibody
    • VAMAS2 antibody
    see all

参考文献 (6)

ab67758 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab67758 は 6 報の論文で使用されています。

  • Wu Z  et al. FOXD3 suppresses epithelial-mesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. Mol Carcinog 60:859-873 (2021). PubMed: 34551139
  • Li R  et al. The transcription factor Foxd3 induces spinal cord ischemia-reperfusion injury by potentiating microRNA-214-dependent inhibition of Kcnk2. Exp Mol Med 52:118-129 (2020). PubMed: 31959866
  • Xu W  et al. FOXD3 Suppresses Tumor-Initiating Features in Lung Cancer via Transcriptional Repression of WDR5. Stem Cells 37:582-592 (2019). PubMed: 30703266
  • Zhao X  et al. Risk-Associated Long Noncoding RNA FOXD3-AS1 Inhibits Neuroblastoma Progression by Repressing PARP1-Mediated Activation of CTCF. Mol Ther 26:755-773 (2018). PubMed: 29398485
  • Huang J & Chen L IL-1β inhibits osteogenesis of human bone marrow-derived mesenchymal stem cells by activating FoxD3/microRNA-496 to repress wnt signaling. Genesis 55:N/A (2017). PubMed: 28509407
  • Guo H  et al. The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation. Genome Biol 17:24 (2016). WB ; Human . PubMed: 26898989

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-FOXD3 antibody - ChIP Grade

 Inconclusive
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Sample
Mouse Tissue lysate - whole (Sciatic nerve)
Specification
Sciatic nerve
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 17 2014

ChIP abreview for Anti-FOXD3 antibody

 Excellent
Abreviews
Application
ChIP
Sample
Chicken Cell lysate - whole cell (Trunk half of the embyo)
Specification
Trunk half of the embyo
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
Chicken embryo trunk samples
Negative control
Not available for sample.
Negative control for QPCR: 3'UTR region of my gene of interest.
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Maneeshi Prasad

Verified customer

投稿 Jun 22 2011

Question

Inquiry: Dear Abcam Team, could you please find out whether one of your FoxD3 antibodies (ab67758 or ab64807) might bind a planarian homologue of FoxD3? Unfortunately I cannot check myself as you don't provide the peptide sequence used for immunization. The sequence of Smed-FoxD3 is MPQAFKDSQTIEQIKNLLRDDDVDVDELKECESDDNIHSESD LDRICSEDKKDVDNKIPMSQERCKSKSHNVKPPYSYIALITMAILRSPQRKLTLSGICEF IMGRFPYYKDRFPAWQNSIRHNLSLNDCFIKIPREPGNPGKGNYWTLDPRSEDMFDNGSF LRRRKRYKRQLPSEMFNHNQSHLIIPPASFRMTIPPNPITVQQNLVNQLLFHQNITKSII PNPNNIIPPLNAIRYPRPFENNFRGHEFIGSLVPPYPHDGRIVIPSTSKTEYNEPINKRC KLNSSEDELSTFQKFSISHIISDDSTDNKTKQSEESYANQFIPQTLRLWPCNPFPVAKPV IKSTWIPPNFIN Thank you in advance for your help. Best regards,

Read More
Answer

Thank you for your inquiry.

I contacted the producing labs of these two antibodies. Although the immunogen sequences are confidential, they did an alignment with the sequence you provided.

Unfortunately, both immunogen sequences did not show any significantly homology with the sequence you provided and I therefore do not recommend these antibodies for your purposes.

I am sorry I did not have a positive answer to your question on this occasion and wish you the best of luck with your research.

Read More

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