AB5060

Anti-FOXC2 抗体

Name: Anti-FOXC2 抗体 (ab5060) | Abcam
Brand: Abcam Limited
SKU: AB5060
Price: 80000 JPY
Availability: InStock

Anti-FOXC2 antibody

Be the first to review this product! Submit a review

(33 Publications)

Goat Polyclonal FOXC2 antibody. Suitable for ChIP, WB and reacts with Mouse, African green monkey, Human, Transfected cell lysate, Transfected cell line samples. Cited in 33 publications. Immunogen corresponding to Synthetic Peptide within Human FOXC2 aa 450 to C-terminus.

別名を表示する

FKHL14, MFH1, FOXC2, Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, Transcription factor FKH-14, MFH-1 protein

2 Images

Supplier Data

Western blot - Anti-FOXC2 antibody (AB5060)

Primary incubation : 1 hour at room temperature.

Negative Control : Human Pancreas lysate.

Lane 1:

Western blot - Anti-FOXC2 antibody (ab5060) at 2 µg/mL

Lane 2:

Western blot - Anti-FOXC2 antibody (ab5060) at 1 µg/mL

Lane 1:

nuclear HEK293 lysate in RIPA buffer at 35 µg

Lane 2:

human pancreas whole cell lysate (negative control) in RIPA buffer at 35 µg

Predicted band size: 53 kDa

Observed band size: 60-70 kDa

true

Unknown

Western blot - Anti-FOXC2 antibody (AB5060)

FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector.

All lanes:

Western blot - Anti-FOXC2 antibody (ab5060)

Predicted band size: 53 kDa

false

Image courtesy of a review by Michal Bell submitted 19 August 2004

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, African green monkey, Mouse

アプリケーション

ChIP, WB

applications

免疫原

Synthetic Peptide within Human FOXC2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99958

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-2 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "African green monkey": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Transfected cell line": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-2 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Transfected cell lysate": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FOXC2 also known as Forkhead Box C2 is a transcription factor weighing approximately 55 kDa. It is a part of the FOX family of transcription factors characterized by a distinct forkhead DNA-binding domain. FOXC2 expresses in various tissues including adipose tissue blood vessels and mesenchymal cells. Its role is to regulate the expression of genes involved in development and differentiation.

Biological function summary

This transcription factor plays a significant role in the development of the cardiovascular and lymphatic systems. It functions as part of a complex network working with other transcription factors to control gene expression that governs vascular remodeling and lymphangiogenesis. Through its activity FOXC2 influences cell fate decisions impacting tissue formation and function during embryogenesis and adult life.

Pathways

FOXC2 participates notably in the TGF-beta signaling pathway. It interacts with SMAD family proteins to influence cellular responses to transforming growth factor-beta signals which are important for cellular proliferation and differentiation. FOXC2 also interfaces with other signaling pathways working in tandem with proteins like Notch and VEGF to modulate angiogenesis and vascular homeostasis.

FOXC2 mutations and dysregulation associate with lymphedema-distichiasis syndrome and various types of cancer. It frequently interacts with other proteins such as VEGFR-3 in these conditions. Mutations affecting FOXC2 can lead to abnormal lymphatic development contributing to fluid retention and limb swelling in lymphedema. In cancer FOXC2 acts as a regulator of cell invasion and metastasis indicating its potential as a therapeutic target.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Transcriptional activator.

See full target information FOXC2

style

left-column

文献 (33)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40464693

2025

A role for pH dynamics regulating transcription factor DNA-binding selectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle P Kisor,Diego Garrido Ruiz,Matthew P Jacobson,Diane L Barber

Nucleic acids research 52:6269-6284 PubMed38634789

2024

Proximal telomeric decompaction due to telomere shortening drives FOXC1-dependent myocardial senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Weiyao Xiong,Wu Zuo,Yuanyuan Shi,Teng Wang,Lingling Chang,Yueheng Wu,Heng Ma,Qian Bian,Alex C Y Chang

Cells 12: PubMed37887278

2023

Aurora Kinase A Regulates Cell Transitions in Glucocorticoid-Induced Bone Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Qiao,Yang Yang,Yan Zhao,Xiuju Wu,Li Zhang,Xinjiang Cai,Jaden Ji,Kristina I Boström,Yucheng Yao

International journal of biological sciences 19:4206-4222 PubMed37705741

2023

FOXC2-AS1/FOXC2 axis mediates matrix stiffness-induced trans-differentiation of hepatic stellate cells into fibrosis-promoting myofibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Liankang Sun,Yue Li,Hao Wang,Xuelian Xiao,Xuenan Luo,Ruida Yang,Jinyan Li,Yifei Ma,Qingguang Liu,Kangsheng Tu,Yu Shi

Cells 12: PubMed37508475

2023

Cell Transitions Contribute to Glucocorticoid-Induced Bone Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Qiao,Xiuju Wu,Yan Zhao,Yang Yang,Li Zhang,Xinjiang Cai,Jocelyn A Ma,Jaden Ji,Karen Lyons,Kristina I Boström,Yucheng Yao

Journal of experimental & clinical cancer research : CR 41:129 PubMed35392966

2022

Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feikai Lin,Xiaoduan Li,Xinjing Wang,Huizhen Sun,Ziliang Wang,Xipeng Wang

Frontiers in oncology 11:634579 PubMed33869020

2021

The FENDRR/FOXC2 Axis Contributes to Multidrug Resistance in Gastric Cancer and Correlates With Poor Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Liu,Zhe Zhang,Yanan Han,Ahui Fan,Haiming Liu,Xiangyuan Zhang,Yanhong Liu,Rugang Zhang,Wanning Liu,Yuanyuan Lu,Daiming Fan,Xiaodi Zhao,Yongzhan Nie

Translational cancer research 9:5268-5280 PubMed35117893

2020

Expression of combined interference of slug and FoxC2 in endometrial carcinoma and its clinicopathological relationship.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Zhu,Mingyang Tang,Ligao Wu

International journal of molecular sciences 21: PubMed32698337

2020

Disease Mutations Identified in Lymphedema Distichiasis Patients Impair Transcriptional Activity and Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Tavian,Sara Missaglia,Sandro Michelini,Paolo Enrico Maltese,Elena Manara,Alvaro Mordente,Matteo Bertelli

eLife 9: PubMed32510325

2020

Shear stimulation of FOXC1 and FOXC2 differentially regulates cytoskeletal activity during lymphatic valve maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Pieter R Norden,Amélie Sabine,Ying Wang,Cansaran Saygili Demir,Ting Liu,Tatiana V Petrova,Tsutomu Kume

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXC2 抗体

Anti-FOXC2 antibody

Western blot - Anti-FOXC2 antibody (AB5060)

Western blot - Anti-FOXC2 antibody (AB5060)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (33)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise