AB5089

Anti-FOXA1 抗体

Name: Anti-FOXA1 抗体 (ab5089) | Abcam
Brand: Abcam Limited
SKU: AB5089
Price: 72000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-FOXA1 antibody

(2 Reviews)

(86 Publications)

Goat Polyclonal FOXA1 antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 86 publications. Immunogen corresponding to Synthetic Peptide within Human FOXA1 aa 450 to C-terminus.

別名を表示する

HNF3A, TCF3A, FOXA1, Hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, Forkhead box protein A1, Transcription factor 3A, TCF-3A

4 Images

Supplier Data

Immunocytochemistry - Anti-FOXA1 antibody (AB5089)

Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% TritonX-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling FOXA1 with ab5089 at 10 ug/ml (1h). Image showing nuclear staining in U-2 OS cells. An Alexa Fluor 488^® secondary antibody was used at 2 ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).

Secondary antibody only control : Unimmunized goat IgG (10 ug/ml) followed by an Alexa Fluor 488^® secondary antibody at 2ug/ml.

Supplier Data

Immunocytochemistry - Anti-FOXA1 antibody (AB5089)

Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling FOXA1 with ab5089 at 10 ug/ml (1h). Image showing nuclear staining in MCF7 cells. An Alexa Fluor 488^® secondary antibody was used at 2 ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).

Secondary antibody only control : Unimmunized goat IgG (10 ug/ml) followed by an Alexa Fluor 488^® secondary antibody at 2ug/ml.

Supplier Data

Western blot - Anti-FOXA1 antibody (AB5089)

Western blot analysis labeling FOXA1 with ab5089.

Lane 1:

Western blot - Anti-FOXA1 antibody (ab5089) at 0.1 µg/mL

Lane 2:

Western blot - Anti-FOXA1 antibody (ab5089) at 0.3 µg/mL

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) nuclear cell lysate at 35 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) nuclear cell lysate at 35 µg

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-FOXA1 antibody (AB5089)

Western blot labeling FOXA1 with ab5089 at 0.1 ug/ml.

All lanes:

Western blot - Anti-FOXA1 antibody (ab5089) at 0.1 µg/mL

All lanes:

Mouse liver lysate at 35 µg

Predicted band size: 49 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, ICC

applications

免疫原

Synthetic Peptide within Human FOXA1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P55317

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "A 1 hour primary incubation at room temperature is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide." }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "A 1 hour primary incubation at room temperature is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide." }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Precipitation Ammonium Sulphate

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FOXA1 also known as Forkhead Box A1 is a transcription factor with a molecular weight of about 49 kDa. It plays a role by binding to specific DNA sequences influencing the expression of genes involved in various cellular processes. This protein is expressed in tissues like liver lungs and prostate. Researchers also refer to FOXA1 as HNF-3α emphasizing its importance in transcription regulation.

Biological function summary

FOXA1 influences the transcriptional control of genes essential for organ development and metabolic regulation. It acts as a pioneer factor opening chromatin structure to enable access for other transcription factors. FOXA1 integrates into complexes that involve histone modification affecting the chromatin state and transcription of target genes. This function is necessary for the proper differentiation and function of several tissues.

Pathways

FOXA1 participates significantly in the androgen receptor signaling pathway and the estrogen receptor signaling pathway. It interacts closely with androgen receptor facilitating the expression of genes that influence cell growth and proliferation. The involvement of FOXA1 in these pathways highlights its critical role in pathways that regulate hormone-responsive cancers and metabolic processes.

FOXA1 shows a strong association with cancers such as prostate cancer and breast cancer. FOXA1's interaction with the androgen receptor links it to prostate cancer progression while its involvement with the estrogen receptor correlates with breast cancer development. Aberrations in its expression or function can disrupt these pathways emphasizing the importance of FOXA1 in cancer biology and its potential as a therapeutic target.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

See full target information FOXA1

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文献 (86)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7118 PubMed40753156

2025

Epiregulon: Single-cell transcription factor activity inference to predict drug response and drivers of cell states.

Applications

Unspecified application

Species

Unspecified reactive species

Tomasz Włodarczyk,Aaron Lun,Diana Wu,Minyi Shi,Xiaofen Ye,Shreya Menon,Shushan Toneyan,Kerstin Seidel,Liang Wang,Jenille Tan,Shang-Yang Chen,Timothy Keyes,Aleksander Chlebowski,Adrian Waddell,Wei Zhou,Yangmeng Wang,Qiuyue Yuan,Yu Guo,Liang-Fu Chen,Bence Daniel,Antonina Hafner,Meng He,Alejandro Chibly,Yuxin Liang,Zhana Duren,Ciara Metcalfe,Marc Hafner,Christian W Siebel,M Ryan Corces,Robert Yauch,Shiqi Xie,Xiaosai Yao

Science advances 9:eadj8442 PubMed38117896

2023

PRKAG2.2 is essential for FoxA1 regulatory T cell differentiation and metabolic rewiring distinct from FoxP3 regulatory T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Mandatori,Yawei Liu,Joana Marturia-Navarro,Mahdieh Hadi,Kristine Henriksen,Jin Zheng,Louise Munk Rasmussen,Salvatore Rizza,Klaus H Kaestner,Shohreh Issazadeh-Navikas

Cancer research communications 3:1788-1799 PubMed37691854

2023

A Druggable FOXA1-Glucocorticoid Receptor Transcriptional Axis Drives Tumor Growth in a Subset of Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

M Dorso,Payal T Patel,Aleksandr Pankov,Jacob A Boyer,Rajesh K Soni,Isabella S Del Priore,Omar Hayatt,Amanda Kulick,Connor J Hagen,Elisa de Stanchina,Melissa R Junttila,Anneleen Daemen,Lori S Friedman,Ronald C Hendrickson,Sarat Chandarlapaty

Cancer research 83:814-829 PubMed36638328

2023

KDM6A Loss Triggers an Epigenetic Switch That Disrupts Urothelial Differentiation and Drives Cell Proliferation in Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Qiu,Vladimir Makarov,Jennifer K Bolzenius,Angela Halstead,Yvonne Parker,Allen Wang,Gopakumar V Iyer,Hannah Wise,Daniel Kim,Varna Thayaparan,Daniel J Lindner,Georges-Pascal Haber,Angela H Ting,Bing Ren,Timothy A Chan,Vivek Arora,David B Solit,Byron H Lee

Nature communications 13:6575 PubMed36323682

2022

FOXA1 repression drives lineage plasticity and immune heterogeneity in bladder cancers with squamous differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua I Warrick,Wenhuo Hu,Hironobu Yamashita,Vonn Walter,Lauren Shuman,Jenna M Craig,Lan L Gellert,Mauro A A Castro,A Gordon Robertson,Fengshen Kuo,Irina Ostrovnaya,Judy Sarungbam,Ying-Bei Chen,Anuradha Gopalan,Sahussapont J Sirintrapun,Samson W Fine,Satish K Tickoo,Kwanghee Kim,Jasmine Thomas,Nagar Karan,Sizhi Paul Gao,Timothy N Clinton,Andrew T Lenis,Timothy A Chan,Ziyu Chen,Manisha Rao,Travis J Hollman,Yanyun Li,Nicholas D Socci,Shweta Chavan,Agnes Viale,Neeman Mohibullah,Bernard H Bochner,Eugene J Pietzak,Min Yuen Teo,Gopa Iyer,Jonathan E Rosenberg,Dean F Bajorin,Matthew Kaag,Suzanne B Merrill,Monika Joshi,Rosalyn Adam,John A Taylor,Peter E Clark,Jay D Raman,Victor E Reuter,Yu Chen,Samuel A Funt,David B Solit,David J DeGraff,Hikmat A Al-Ahmadie

Cancer research 82:3673-3686 PubMed35950920

2022

A Distinct Chromatin State Drives Therapeutic Resistance in Invasive Lobular Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Agostina Nardone,Xintao Qiu,Sandor Spisak,Zsuzsanna Nagy,Ariel Feiglin,Avery Feit,Gabriela Cohen Feit,Yingtian Xie,Alba Font-Tello,Cristina Guarducci,Francisco Hermida-Prado,Sudeepa Syamala,Klothilda Lim,Miguel Munoz Gomez,Matthew Pun,MacIntosh Cornwell,Weihan Liu,Aysegul Ors,Hisham Mohammed,Paloma Cejas,Jane B Brock,Matthew L Freedman,Eric P Winer,Xiaoyong Fu,Rachel Schiff,Henry W Long,Otto Metzger Filho,Rinath Jeselsohn

Cancer discovery 12:2074-2097 PubMed35754340

2022

Drug-Induced Epigenomic Plasticity Reprograms Circadian Rhythm Regulation to Drive Prostate Cancer toward Androgen Independence.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Linder,Marlous Hoogstraat,Suzan Stelloo,Nils Eickhoff,Karianne Schuurman,Hilda de Barros,Maartje Alkemade,Elise M Bekers,Tesa M Severson,Joyce Sanders,Chia-Chi Flora Huang,Tunc Morova,Umut Berkay Altintas,Liesbeth Hoekman,Yongsoo Kim,Sylvan C Baca,Martin Sjöström,Anniek Zaalberg,Dorine C Hintzen,Jeroen de Jong,Roelof J C Kluin,Iris de Rink,Claudia Giambartolomei,Ji-Heui Seo,Bogdan Pasaniuc,Maarten Altelaar,René H Medema,Felix Y Feng,Amina Zoubeidi,Matthew L Freedman,Lodewyk F A Wessels,Lisa M Butler,Nathan A Lack,Henk van der Poel,Andries M Bergman,Wilbert Zwart

Nature communications 12:6636 PubMed34789735

2021

Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan J Geusz,Allen Wang,Dieter K Lam,Nicholas K Vinckier,Konstantinos-Dionysios Alysandratos,David A Roberts,Jinzhao Wang,Samy Kefalopoulou,Araceli Ramirez,Yunjiang Qiu,Joshua Chiou,Kyle J Gaulton,Bing Ren,Darrell N Kotton,Maike Sander

Urologia internationalis 107:58-63 PubMed34706362

2021

Expression of FOXA1 Is Associated with the Tumor-Infiltrating M2 Macrophage, Cytotoxic T Lymphocyte, and Effect of Chemotherapy in Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yiduo Wang,Huan Qu,Bin Xu,Jianping Wu,Kai Lu,Chunhui Liu,Shuqiu Chen,Ming Chen

BMC cancer 21:768 PubMed34215221

2021

Differential gene expression identifies a transcriptional regulatory network involving ER-alpha and PITX1 in invasive epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yichao Li,Sushil K Jaiswal,Rupleen Kaur,Dana Alsaadi,Xiaoyu Liang,Frank Drews,Julie A DeLoia,Thomas Krivak,Hanna M Petrykowska,Valer Gotea,Lonnie Welch,Laura Elnitski

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXA1 抗体

Anti-FOXA1 antibody

Immunocytochemistry - Anti-FOXA1 antibody (AB5089)

Immunocytochemistry - Anti-FOXA1 antibody (AB5089)

Western blot - Anti-FOXA1 antibody (AB5089)

Western blot - Anti-FOXA1 antibody (AB5089)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (86)

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組合せ製品

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Secondary Antibodies

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Abcam product promise