Anti-FOXA1 抗体

• WB

Lab

Western blot - Anti-FOXA1 antibody (AB23738)

Lanes 1 - 4 : Merged signal (red and green). Green - ab23738 observed at 49 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab23738 was shown to recognize FOXA1 in wild-type HAP1 cells as signal was lost at the expected MW in FOXA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FOXA1 knockout samples were subjected to SDS-PAGE. ab23738 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.7 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FOXA1 antibody (ab23738) at 0.7 µg/mL

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

FOXA1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HepG2 whole cell lysate at 20 µg

Lane 4:

A549 whole cell lysate at 20 µg

Predicted band size: 49 kDa

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• WB

AbReview15258****

Western blot - Anti-FOXA1 antibody (AB23738)

All lanes:

Western blot - Anti-FOXA1 antibody (ab23738) at 1/1000 dilution

Lane 1:

Lysate prepared from human LNCaP cells at 20 µg

Lane 2:

Lysate prepared from MCF7 cells at 20 µg

Lane 3:

Lysate prepared from human LNCaP cells with ethanol as vehicle control at 20 µg

Lane 4:

Lysate prepared from human LNCaP cells with 100 nM DHT at 20 µg

Secondary

All lanes:

HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution

Predicted band size: 49 kDa

Observed band size: 51 kDa

true

Exposure time: 2s

This image is a courtesy of Anonymous Abreview

• WB

Unknown

Western blot - Anti-FOXA1 antibody (AB23738)

All lanes:

Western blot - Anti-FOXA1 antibody (ab23738) at 1 µg/mL

All lanes:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 31 kDa,51 kDa

false

• WB

Unknown

Western blot - Anti-FOXA1 antibody (AB23738)

All lanes:

Western blot - Anti-FOXA1 antibody (ab23738) at 1 µg/mL

All lanes:

Recombinant Human FOXA1 protein (ab98301) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 49 kDa

true

Exposure time: 10s