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リコンビナントRabMAb

Anti-Fos B 抗体 [EPR15905] (ab184938)

Reviews (2) Submit a question References (27)
Western blot - Anti-Fos B antibody [EPR15905] (ab184938)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fos B antibody [EPR15905] (ab184938)
  • Western blot - Anti-Fos B antibody [EPR15905] (ab184938)
  • Western blot - Anti-Fos B antibody [EPR15905] (ab184938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)
  • Immunoprecipitation - Anti-Fos B antibody [EPR15905] (ab184938)
  • Anti-Fos B antibody [EPR15905] (ab184938)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15905] to Fos B
  • Suitable for: IP, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Carrier Free

製品の概要

  • 製品名

    Anti-Fos B antibody [EPR15905]
    Fos B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15905] to Fos B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, ICC/IF, WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr; NIH/3T3 and C6 whole cell lysate treated with serum, starved overnight, then serum stimulated for 4hr. IHC-P: Mouse hippocampus, mouse kidney, rat hippocampus and rat kidney tissues. ICC/IF: HeLa cells. IP: HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184938の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/50.
ICC/IF
1/500.
WB
1/10000. Detects a band of approximately 38, 48 kDa (predicted molecular weight: 36 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IP
1/50.
ICC/IF
1/500.
WB
1/10000. Detects a band of approximately 38, 48 kDa (predicted molecular weight: 36 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

画像

  • Western blot - Anti-Fos B antibody [EPR15905] (ab184938)
    Western blot - Anti-Fos B antibody [EPR15905] (ab184938)
    All lanes : Anti-Fos B antibody [EPR15905] (ab184938) at 1/10000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 38,48 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    FosB protein exists as two isoforms: full-length FosB (48KD) and a truncated form, FosB2 (38KD, Delta FosB) which lacks the carboxyl-terminal 101 amino acids.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fos B antibody [EPR15905] (ab184938)
    Immunocytochemistry/ Immunofluorescence - Anti-Fos B antibody [EPR15905] (ab184938)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, untreated or treated with 12-o-tetradecanoyl phorbol 13-acetate (TPA) (200nM) for 4h, labeling Fos B with ab184938 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HeLa cells. The expression was increaed after treatment with 12-o-tetradecanoyl phorbol 13-acetate (TPA) (200nM) for 4h.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184938 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Western blot - Anti-Fos B antibody [EPR15905] (ab184938)
    Western blot - Anti-Fos B antibody [EPR15905] (ab184938)
    All lanes : Anti-Fos B antibody [EPR15905] (ab184938) at 1/10000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
    Lane 2 : NIH/3T3 whole cell lysate treated with serum, starved overnight, then serum stimulated for 4hr

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 38,48 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    FosB protein exists as two isoforms: full-length FosB (48KD) and a truncated form, FosB2 (38KD, Delta FosB) which lacks the carboxyl-terminal 101 amino acids

  • Western blot - Anti-Fos B antibody [EPR15905] (ab184938)
    Western blot - Anti-Fos B antibody [EPR15905] (ab184938)
    All lanes : Anti-Fos B antibody [EPR15905] (ab184938) at 1/10000 dilution

    Lane 1 : Untreated C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : C6 whole cell lysate treated with serum, starved overnight, then serum stimulated for 4hr

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 38,48 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    FosB protein exists as two isoforms: full-length FosB (48KD) and a truncated form, FosB2 (38KD, Delta FosB) which lacks the carboxyl-terminal 101 amino acids

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse hippocampus is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat hippocampus is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on rat kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Fos B antibody [EPR15905] (ab184938)
    Immunoprecipitation - Anti-Fos B antibody [EPR15905] (ab184938)

    Fos B was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr using ab184938 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab184938 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr, 10 µg (Input).

    Lane 2: ab184938 IP in HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184938 in HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-Fos B antibody [EPR15905] (ab184938)
    Anti-Fos B antibody [EPR15905] (ab184938)

参考文献 (27)

ab184938 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184938 は 27 報の論文で使用されています。

  • Bollinger JL  et al. Repeated Activation of Pyramidal Neurons in the Prefrontal Cortex Alters Microglial Phenotype in Male Mice. J Pharmacol Exp Ther 388:715-723 (2024). PubMed: 38129124
  • Zhang Z  et al. PHF5A facilitates the development and progression of gastric cancer through SKP2-mediated stabilization of FOS. J Transl Med 21:5 (2023). PubMed: 36609277
  • Woodward E  et al. Sex-Specific Timelines for Adaptations of Prefrontal Parvalbumin Neurons in Response to Stress and Changes in Anxiety- and Depressive-Like Behaviors. eNeuro 10:N/A (2023). PubMed: 36808099
  • Kawamura A & Nishiyama M Deletion of the autism-related gene Chd8 alters activity-dependent transcriptional responses in mouse postmitotic neurons. Commun Biol 6:593 (2023). PubMed: 37268684
  • Hosoya T  et al. Chondroprotective effects of CDK4/6 inhibition via enhanced ubiquitin-dependent degradation of JUN in synovial fibroblasts. Rheumatology (Oxford) 61:3427-3438 (2022). PubMed: 34849618
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Fos B antibody [EPR15905]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Mouse brain (dentate gyrus of the hippocampus))
Antigen retrieval step
None
Permeabilization
Yes - 0.3% triton X 100
Specification
Mouse brain (dentate gyrus of the hippocampus)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Liz Kirby

Verified customer

投稿 Jun 26 2024

Immunohistochemistry (Frozen sections) abreview for Anti-Fos B antibody [EPR15905]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 06 2016

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