Anti-Folate Binding Protein/FBP 抗体 (ab67422)
Key features and details
- Rabbit polyclonal to Folate Binding Protein/FBP
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Folate Binding Protein/FBP antibody
Folate Binding Protein/FBP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Folate Binding Protein/FBP -
由来種
Rabbit -
特異性
From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse cerebellum tissue lysate, JAR and HeLa cell lysates. ICC/IF: HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67422の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). Please note, we have been advised by some customers that ab67422 is unable to detect the human version of this protein in western blot. Please contact our scientific support services if you have any queries regarding this antibody.
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| IHC-P | (1) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). Please note, we have been advised by some customers that ab67422 is unable to detect the human version of this protein in western blot. Please contact our scientific support services if you have any queries regarding this antibody. |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. -
組織特異性
Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. -
関連疾患
Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. -
配列類似性
Belongs to the folate receptor family. -
翻訳後修飾
Eight disulfide bonds are present.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. -
細胞内局在
Cell membrane. Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 2348 Human
- Entrez Gene: 171049 Rat
- Omim: 136430 Human
- SwissProt: P15328 Human
- SwissProt: P35846 Mouse
- Unigene: 73769 Human
- Unigene: 2135 Mouse
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別名
- adult antibody
- Adult folate binding protein antibody
- Adult folate-binding protein antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody (ab67422)
IHC image of Folate Binding Protein/FBP staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67422, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - Anti-Folate Binding Protein/FBP antibody (ab67422)All lanes : Anti-Folate Binding Protein/FBP antibody (ab67422) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FOLR1 knockout HeLa cell lysate
Lane 3 : JAR cell lysate
Lane 4 : MDA-MB-231 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab67422 observed at 38 kDa. Red - loading control, ab7291 observed at 52 kDa.
ab67422 Anti-Folate Binding Protein/FBP antibody was shown to specifically react with Folate Binding Protein/FBP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264921 (knockout cell lysate ab257270) was used. Wild-type and Folate Binding Protein/FBP knockout samples were subjected to SDS-PAGE. ab67422 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Folate Binding Protein/FBP antibody (ab67422)Anti-Folate Binding Protein/FBP antibody (ab67422) at 1/1 dilution + Cerebellum Mouse Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa (possible cleavage fragment), 22 kDa (possible cleavage fragment)
We hypothesize that the 28, 22 and 16 kDa bands represent the propeptide with signal sequence, propeptide without signal sequence and mature protein, respectively. -
Immunocytochemistry/ Immunofluorescence - Anti-Folate Binding Protein/FBP antibody (ab67422)ab67422 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab67422 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab67422 は 9 報の論文で使用されています。
- Khan S et al. Administration of prosaposin-derived neurotrophic factor to neural tube defects facilitates regeneration and restores neurological functions. iScience 26:106277 (2023). PubMed: 37153447
- Yazaki S et al. High expression of folate receptor alpha is associated with poor prognosis in patients with cervical cancer. J Gynecol Oncol 33:e82 (2022). PubMed: 36245230
- Guzik P et al. Promising potential of [177Lu]Lu-DOTA-folate to enhance tumor response to immunotherapy-a preclinical study using a syngeneic breast cancer model. Eur J Nucl Med Mol Imaging 48:984-994 (2021). PubMed: 33078260
- Alam C et al. Upregulation of reduced folate carrier by vitamin D enhances brain folate uptake in mice lacking folate receptor alpha. Proc Natl Acad Sci U S A 116:17531-17540 (2019). PubMed: 31405972
- Martin JB et al. Folic acid modifies the shape of epithelial cells during morphogenesis via a Folr1 and MLCK dependent mechanism. Biol Open 8:N/A (2019). PubMed: 30670450
- Siwowska K et al. Folate Receptor-Positive Gynecological Cancer Cells: In Vitro and In Vivo Characterization. Pharmaceuticals (Basel) 10:N/A (2017). PubMed: 28809784
- Meredith M et al. Growing Mouse Oocytes Transiently Activate Folate Transport via Folate Receptors As They Approach Full Size. Biol Reprod 94:125 (2016). PubMed: 27122634
- Ocak M et al. Folate receptor-targeted multimodality imaging of ovarian cancer in a novel syngeneic mouse model. Mol Pharm 12:542-53 (2015). PubMed: 25536192
- Castiglioni V et al. Immunohistochemical Characterization of a Renal Nephroblastoma in a Trp53-mutant and Prolyl Isomerase 1-deficient Mouse. J Toxicol Pathol 26:423-7 (2013). PubMed: 24526816