Anti-FNTB 抗体 [EPR4707] (ab109625)
![Western blot - Anti-FNTB antibody [EPR4707] (ab109625)](https://www.abcam.co.jp/ps/products/109/ab109625/Images/ab109625-122574-anti-fntb-antibody-epr4707-western-blot.jpg "Western blot - Anti-FNTB antibody [EPR4707] (ab109625)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4707] to FNTB
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FNTB antibody [EPR4707]
FNTB 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4707] to FNTB -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- JAR, A431, U87-MG and A375 cell lysates, Human breast tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4707 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109625の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 49 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
追加情報
Is unsuitable for ICC/IF.
ターゲット情報
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機能
Catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding. -
配列類似性
Belongs to the protein prenyltransferase subunit beta family.
Contains 5 PFTB repeats. - Information by UniProt
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参照データベース
- Entrez Gene: 2342 Human
- Omim: 134636 Human
- SwissProt: P49356 Human
- Unigene: 632345 Human
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別名
- CAAX farnesyltransferase subunit beta antibody
- EC 2.5.1.58 antibody
- EC=2.5.1.58 antibody
see all
画像
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Western blot - Anti-FNTB antibody [EPR4707] (ab109625)All lanes : Anti-FNTB antibody [EPR4707] (ab109625) at 1/10000 dilution
Lane 1 : JAR cell lysate
Lane 2 : A431 cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 49 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FNTB antibody [EPR4707] (ab109625)](https://www.abcam.co.jp/ps/products/109/ab109625/Images/ab109625-122573-anti-fntb-antibody-epr4707-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FNTB antibody [EPR4707] (ab109625)Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109625 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-FNTB antibody [EPR4707] (ab109625)](https://www.abcam.co.jp/ps/products/109/ab109625/Images/ab109625-3-benefits-of-recombinant-antibodies.png)
Anti-FNTB antibody [EPR4707] (ab109625)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab109625 は 4 報の論文で使用されています。
- Hadfield JD et al. Cell Synchronization Techniques for Studying Mitosis. Methods Mol Biol 2579:73-86 (2022). PubMed: 36045199
- Pinner AL et al. Protein expression of prenyltransferase subunits in postmortem schizophrenia dorsolateral prefrontal cortex. Transl Psychiatry 10:3 (2020). PubMed: 32066669
- Makhov P et al. CRISPR/Cas9 genome-wide loss-of-function screening identifies druggable cellular factors involved in sunitinib resistance in renal cell carcinoma. Br J Cancer 123:1749-1756 (2020). PubMed: 32968206
- Liang J et al. Inhibition of the mevalonate pathway enhances cancer cell oncolysis mediated by M1 virus. Nat Commun 9:1524 (2018). PubMed: 29670091