Anti-FNTB 抗体 [EPR4706] (ab109748)
![Western blot - Anti-FNTB antibody [EPR4706] (ab109748)](https://www.abcam.co.jp/ps/products/109/ab109748/Images/ab109748-253954-anti-fntb-antibody-epr4706-western-blot.jpg "Western blot - Anti-FNTB antibody [EPR4706] (ab109748)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4706] to FNTB
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-FNTB antibody [EPR4706]
FNTB 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4706] to FNTB -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details
適用なし: IHC-P -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: JAR, HepG2, K562, SH-SY5Y and C6 cell lysates. ICC/IF: K562 cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4706 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109748の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500.
For unpurified use at 1/50 - 1/100. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
|
| 特記事項 |
|---|
|
ICC/IF
1/500. For unpurified use at 1/50 - 1/100. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa. |
ターゲット情報
-
機能
Catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding. -
配列類似性
Belongs to the protein prenyltransferase subunit beta family.
Contains 5 PFTB repeats. - Information by UniProt
-
参照データベース
- Entrez Gene: 2342 Human
- Entrez Gene: 64511 Rat
- Omim: 134636 Human
- SwissProt: P49356 Human
- SwissProt: Q02293 Rat
- Unigene: 632345 Human
- Unigene: 8873 Rat
-
別名
- CAAX farnesyltransferase subunit beta antibody
- EC 2.5.1.58 antibody
- EC=2.5.1.58 antibody
see all
画像
-
Western blot - Anti-FNTB antibody [EPR4706] (ab109748)Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution (purified) + C6 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-FNTB antibody [EPR4706] (ab109748)](https://www.abcam.co.jp/ps/products/109/ab109748/Images/ab109748-253953-anti-fntb-antibody-epr4706-western-blot.jpg)
Western blot - Anti-FNTB antibody [EPR4706] (ab109748)All lanes : Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution (purified)
Lane 1 : JAR whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunocytochemistry/ Immunofluorescence - Anti-FNTB antibody [EPR4706] (ab109748)](https://www.abcam.co.jp/ps/products/109/ab109748/Images/ab109748-253871-anti-fntb-antibody-epr4706-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FNTB antibody [EPR4706] (ab109748)Immunocytochemistry/Immunofluorescence analysis of K562 cells labelling FNTB with purified ab109748 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
-
![Western blot - Anti-FNTB antibody [EPR4706] (ab109748)](https://www.abcam.co.jp/ps/products/109/ab109748/Images/ab109748-122741-anti-fntb-antibody-epr4706-western-blot.jpg)
Western blot - Anti-FNTB antibody [EPR4706] (ab109748)All lanes : Anti-FNTB antibody [EPR4706] (ab109748) at 1/1000 dilution (unpurified)
Lane 1 : JAR cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa -
![Anti-FNTB antibody [EPR4706] (ab109748)](https://www.abcam.co.jp/ps/products/109/ab109748/Images/ab109748-5-benefits-of-recombinant-antibodies.png)
Anti-FNTB antibody [EPR4706] (ab109748)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab109748 は 1 報の論文で使用されています。
- Jiang D et al. Expression of key enzymes in the mevalonate pathway are altered in monocrotaline-induced pulmonary arterial hypertension in rats. Mol Med Rep 16:9593-9600 (2017). PubMed: 29039598