Anti-FMO3 抗体 [EPR6968] (ab126711)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6968] to FMO3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-FMO3 antibody [EPR6968]
FMO3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6968] to FMO3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human FMO3 aa 300-400. The exact sequence is proprietary.
Database link: P31513 -
ポジティブ・コントロール
- WB: Human fetal liver, human fetal kidney, mouse kidney, mouse liver, rat kidney and rat liver tissue lysates. IHC-P: Human, mouse and rat liver tissues. ICC/IF: HepG2 cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 2.40 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6968 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
- Mouse liver tissue lysate - total protein (ab29301)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Mouse kidney tissue lysate - total protein (0 days) (ab7261)
- Mouse kidney tissue lysate (14 days) (ab7262)
- Mouse kidney tissue lysate (7 days) (ab7263)
- Mouse liver tissue lysate - total protein (7 days) (ab7266)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126711の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (1) |
1/150.
|
WB | (2) |
1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa).
For unpurified use at 1/1000 - 1/10000. |
IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
---|
ICC/IF
1/150. |
WB
1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa). For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
機能
Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds. -
組織特異性
Liver. -
関連疾患
Defects in FMO3 are the cause of trimethylaminuria (TMAU) [MIM:602079]; also known as fish-odor syndrome. TMAU is an inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino-trimethylamine (TMA) derived from foodstuffs. Such individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine. -
配列類似性
Belongs to the FMO family. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 2328 Human
- Entrez Gene: 14262 Mouse
- Entrez Gene: 84493 Rat
- Omim: 136132 Human
- SwissProt: P31513 Human
- SwissProt: P97501 Mouse
- SwissProt: Q9EQ76 Rat
- Unigene: 445350 Human
see all -
別名
- Dimethylaniline monooxygenase [N oxide forming] 3 antibody
- Dimethylaniline monooxygenase [N-oxide-forming] 3 antibody
- Dimethylaniline monooxygenase 3 antibody
see all
画像
-
All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/5000 dilution (purified)
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
-
All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/5000 dilution (purified)
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
-
All lanes : Anti-FMO3 antibody [EPR6968] (ab126711) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling FMO3 with purified ab126711 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody [EPR6968] (ab126711)
Immunohistochemitsy (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FMO3 with unpurified ab126711 at a 1/500 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling FMO3 with purified ab126711 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (7)
ab126711 は 7 報の論文で使用されています。
- Gan G et al. Gut microbiota may mediate the impact of chronic apical periodontitis on atherosclerosis in apolipoprotein E-deficient mice. Int Endod J 56:53-68 (2023). PubMed: 36208054
- Liu A et al. Trimethylamine N-oxide promotes atherosclerosis via regulating the enriched abundant transcript 1/miR-370-3p/signal transducer and activator of transcription 3/flavin-containing monooxygenase-3 axis. Bioengineered 13:1541-1553 (2022). PubMed: 34923910
- Huang S et al. Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism. Front Cell Dev Biol 9:630188 (2021). PubMed: 33644069
- Bai S et al. Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype. Front Mol Biosci 8:650604 (2021). PubMed: 34277700
- Li L et al. Fructus Ligustri Lucidi preserves bone quality through the regulation of gut microbiota diversity, oxidative stress, TMAO and Sirt6 levels in aging mice. Aging (Albany NY) 11:9348-9368 (2019). PubMed: 31715585
- Astafev AA et al. Calorie restriction effects on circadian rhythms in gene expression are sex dependent. Sci Rep 7:9716 (2017). PubMed: 28851928
- Liao BM et al. Flavin-Containing Monooxygenase 3 Reduces Endoplasmic Reticulum Stress in Lipid-Treated Hepatocytes. Mol Endocrinol 30:417-28 (2016). PubMed: 26886171