Anti-Flt3 ligand/Flt3L 抗体 (ab9688)
Key features and details
- Rabbit polyclonal to Flt3 ligand/Flt3L
- Suitable for: Flow Cyt, WB, Neutralising, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-Flt3 ligand/Flt3L antibody
Flt3 ligand/Flt3L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Flt3 ligand/Flt3L -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, WB, Neutralising, Sandwich ELISAmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human Flt3 ligand/Flt3L aa 1-200. Highly pure>98%. Produced in E. coli.
Database link: P49771 -
特記事項
hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
No preservative, sterile filtered -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9688の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration. PubMed: 22897934
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
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| Neutralising |
Use at an assay dependent concentration.
Yields one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/ml) |
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| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
In conjunction with ab84261 rabbit polyclonal to Flt3 ligand/Flt3L (Biotin) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand/Flt3L. |
| 特記事項 |
|---|
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Flow Cyt
Use at an assay dependent concentration. PubMed: 22897934 |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Neutralising
Use at an assay dependent concentration. Yields one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/ml) |
|
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. In conjunction with ab84261 rabbit polyclonal to Flt3 ligand/Flt3L (Biotin) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand/Flt3L. |
ターゲット情報
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機能
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2323 Human
- Omim: 600007 Human
- SwissProt: P49771 Human
- Unigene: 428 Human
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別名
- FL antibody
- Flt 3 ligand antibody
- Flt 3L antibody
see all
画像
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Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (ab9688)
To detect human Flt3 ligand/Flt3L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84261 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Human Flt3-ligand (ab9688) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Flt3 ligand/Flt3L.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab9688 は 3 報の論文で使用されています。
- Yuan X et al. Mesenchymal stem cell therapy induces FLT3L and CD1c+ dendritic cells in systemic lupus erythematosus patients. Nat Commun 10:2498 (2019). PubMed: 31175312
- Barry KC et al. A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments. Nat Med 24:1178-1191 (2018). PubMed: 29942093
- Patel ES et al. Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation. BMC Immunol 13:46 (2012). Flow Cyt ; Human . PubMed: 22897934
