AB9688

Anti-Flt3 ligand/Flt3L 抗体

Name: Anti-Flt3 ligand/Flt3L 抗体 (ab9688) | Abcam
Brand: Abcam Limited
SKU: AB9688
Price: 49000 JPY
Availability: InStock

Anti-Flt3 ligand/Flt3L antibody

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(3 Publications)

Rabbit Polyclonal Flt3 ligand/Flt3L antibody. Suitable for Flow Cyt, WB, sELISA and reacts with Recombinant fragment, Human, Recombinant full length protein - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FLT3LG aa 1-200.

別名を表示する

Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG

1 Images

Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (AB9688)

sELISA

Supplier Data

Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (AB9688)

To detect human Flt3 ligand/Flt3L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84261 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Human Flt3-ligand (ab9688) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Flt3 ligand/Flt3L.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt, WB, sELISA

applications

免疫原

Recombinant Fragment Protein within Human FLT3LG aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P49771

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant fragment": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.5-2 µg/mL", "sELISA-species-notes": "In conjunction with <a href='/products/primary-antibodies/biotin-flt3-ligand-flt3l-antibody-ab84261'>ab84261</a> rabbit polyclonal to Flt3 ligand/Flt3L (Biotin) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand/Flt3L." } } }

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製品の詳細

hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells

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出荷温度及び保存条件

製品の状態

Lyophilized

再構成

reconstitute with water at 200µL

精製方法

Affinity purification Immunogen

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Flt3 ligand (Flt3L) also known as Flt3-L Flt3 ligand protein or flt-3 ligand protein is a small cytokine with a molecular mass of approximately 18 to 19 kDa. It acts as a growth factor by binding to the Flt3 receptor tyrosine kinase on the surface of hematopoietic progenitor cells. Flt3L is expressed in various tissues including the bone marrow where it plays a significant role in hematopoiesis. It is critical in dendritic cell and B cell development impacting the immune response mechanism.

Biological function summary

The Flt3 ligand plays an essential role in the immune system. It is a monomeric cytokine that induces proliferation of early lineage cells within the hematopoietic system. Flt3L does not function as part of a complex but acts independently to stimulate the proliferation differentiation and survival of hematopoietic cells. By interacting directly with its receptor it significantly influences the innate and adaptive immune systems through the maturation of dendritic and B cells.

Pathways

The Flt3 ligand is involved in hematopoietic signaling pathways primarily the PI3K-Akt signaling pathway. This pathway is essential for cell survival and growth where Flt3L aids in transmitting growth signals necessary for hematopoietic cells. Flt3L also interacts with proteins such as FLT3 receptor and influences the downstream signaling cascade affecting cell fate decisions in hematopoiesis and immune cell maturation.

Flt3 ligand is connected to leukemia particularly acute myeloid leukemia (AML) due to dysregulation of the FLT3 signaling pathway. Mutations in the FLT3 receptor can lead to uncontrolled cell proliferation contributing to tumorigenesis. Additionally Flt3L plays a role in autoimmune disorders by influencing immune cell development. It is closely connected with the FLT3 receptor and other tyrosine kinases which when mutated can exacerbate disease pathology through abnormal cell signaling and proliferation.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Required for the development of B cells, and dendritic cells (DCs).

See full target information FLT3LG

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:2498 PubMed31175312

2019

Mesenchymal stem cell therapy induces FLT3L and CD1c dendritic cells in systemic lupus erythematosus patients.

Applications

Unspecified application

Species

Unspecified reactive species

Xinran Yuan,Xiaodong Qin,Dandan Wang,Zhuoya Zhang,Xiaojun Tang,Xiang Gao,Wanjun Chen,Lingyun Sun

Nature medicine 24:1178-1191 PubMed29942093

2018

A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin C Barry,Joy Hsu,Miranda L Broz,Francisco J Cueto,Mikhail Binnewies,Alexis J Combes,Amanda E Nelson,Kimberly Loo,Raj Kumar,Michael D Rosenblum,Michael D Alvarado,Denise M Wolf,Dusan Bogunovic,Nina Bhardwaj,Adil I Daud,Patrick K Ha,William R Ryan,Joshua L Pollack,Bushra Samad,Saurabh Asthana,Vincent Chan,Matthew F Krummel

BMC immunology 13:46 PubMed22897934

2012

Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation.

Applications

Flow Cyt

Species

Human

Ekta S Patel,Starlyn Okada,Kevin Hachey,Li-jun Yang,Scott K Durum,Jan S Moreb,Lung-Ji Chang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Flt3 ligand/Flt3L 抗体

Anti-Flt3 ligand/Flt3L antibody

Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (AB9688)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

再構成

精製方法

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

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組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise