Anti-Flt3 / CD135 抗体 [EPR22232-318] - BSA and Azide free (ab245824)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22232-318] to Flt3 / CD135 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Flt3 / CD135 antibody [EPR22232-318] - BSA and Azide free
Flt3 / CD135 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22232-318] to Flt3 / CD135 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: His-tagged human Flt3 / CD135 active protein(aa27-541); THP-1 whole cell lysate. IP: THP-1 whole cell lysate.
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特記事項
ab245824 is the carrier-free version of ab245116.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22232-318 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245824の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 160, 130 kDa (predicted molecular weight: 113 kDa).
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IP |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 160, 130 kDa (predicted molecular weight: 113 kDa). |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Receptor for the FL cytokine. Has a tyrosine-protein kinase activity. -
組織特異性
Bone marrow cells. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 protein kinase domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2322 Human
- Omim: 136351 Human
- SwissProt: P36888 Human
- Unigene: 507590 Human
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別名
- CD 135 antibody
- CD135 antibody
- CD135 antigen antibody
see all
画像
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All lanes : Anti-Flt3 / CD135 antibody [EPR22232-318] (ab245116) at 1/1000 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : FLT3 knockout THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Predicted band size: 113 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Flt3 / CD135 antibody [EPR22232-318] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab245116 was shown to bind specifically to Flt3 / CD135. A band was observed at 130 kDa in wild-type THP-1 cell lysates with no signal observed at this size in FLT3 knockout cell line ab282857 (knockout cell lysate ab283225). The band observed at 60 kDa has not been identified. To generate this image, wild-type and FLT3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245116).
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Flt3 / CD135 was immunoprecipitated from 0.35 mg of THP-1 (human monocytic leukemia cell line) whole cell lysate with ab245116 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245116 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: THP-1 whole cell lysate 10 μg (Input).
Lane 2: ab245116 IP in THP-1 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245116 in THP-1 whole cell lysate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 minute.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245116).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab245824 は論文での使用が確認できていません。