Anti-FLK1 抗体 [Clone 10] (ab24313)
Key features and details
- Mouse monoclonal [Clone 10] to FLK1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
製品の概要
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製品名
Anti-FLK1 antibody [Clone 10] -
製品の詳細
Mouse monoclonal [Clone 10] to FLK1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Recombinant fragment corresponding to Saccharomyces cerevisiae FLK1 aa 1-200.
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ポジティブ・コントロール
- Mav108 cells expressing recombinant FLK1.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
Clone 10 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24313の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa).
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特記事項 |
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WB
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa). |
ターゲット情報
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関連性
Yeast TUP1 Ssn6p repressor complex represents a novel paradigm for transcriptional repression and for the role of chromatin in repression. TUP1 and SSN6 are involved in repression of several diverse families of genes in yeast, including cell type specific genes regulated by the alpha2 and a1/alpha2 repressors. Ssn6 Tupl interacts with class I histone deacetylases required for repression, and recruitment of yeast Tuplp Ssn6p repressor is associated with localised decreases in histone acetylation. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 850445 Saccharomyces cerevisiae
- SwissProt: P16649 Saccharomyces cerevisiae
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別名
- FLK1 antibody
- Flocculation suppressor protein antibody
- Repressor AER2 antibody
see all
画像
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All lanes : Anti-FLK1 antibody [Clone 10] (ab24313)
All lanes : Saccharomyces cerevisiae whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Sheep anti-Mouse polyclonal HRP conjugate at 100 %
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
データシートおよび資料
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Datasheet download
参考文献 (1)
ab24313 は 1 報の論文で使用されています。
- Mei B et al. The regulatory mechanism and biological significance of the Snail-miR590-VEGFR-NRP1 axis in the angiogenesis, growth and metastasis of gastric cancer. Cell Death Dis 11:241 (2020). PubMed: 32303680