Anti-FLJ35220 抗体 (ab69400)
Key features and details
- Mouse polyclonal to FLJ35220
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FLJ35220 antibody -
製品の詳細
Mouse polyclonal to FLJ35220 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human FLJ35220 aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- FLJ35220 transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69400の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
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| WB |
1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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| 特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
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WB
1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
ターゲット情報
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関連性
This gene encodes hypothetical protein FLJ35220 -
参照データベース
- Entrez Gene: 284131 Human
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別名
- hypothetical protein FLJ35220 antibody
- hypothetical protein LOC284131 antibody
- MGC74455 antibody
画像
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Western blot - Anti-FLJ35220 antibody (ab69400)All lanes : Anti-FLJ35220 antibody (ab69400) at 1/500 dilution
Lane 1 : FLJ35220 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-FLJ35220 antibody (ab69400)ab69400 at 10µg/ml staining FLJ35220 in Hela cells by ICC/IF.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab69400 は 3 報の論文で使用されています。
- Kong XY et al. Endonuclease V Regulates Atherosclerosis Through C-C Motif Chemokine Ligand 2-Mediated Monocyte Infiltration. J Am Heart Assoc 10:e020656 (2021). PubMed: 34259011
- Berges N et al. Complex alternative splicing of human Endonuclease V mRNA, but evidence for only a single protein isoform. PLoS One 14:e0225081 (2019). PubMed: 31703097
- Nawaz MS et al. Regulation of Human Endonuclease V Activity and Relocalization to Cytoplasmic Stress Granules. J Biol Chem 291:21786-21801 (2016). PubMed: 27573237
