Anti-FLJ20530 抗体 (ab18050)
Key features and details
- Rabbit polyclonal to FLJ20530
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FLJ20530 antibody -
製品の詳細
Rabbit polyclonal to FLJ20530 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
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免疫原
Synthetic peptide corresponding to Human FLJ20530. Synthetic peptide (Human) - the immunogen lies in the region between residues 200 and 250 of human FLJ20530/ KAONASHI1 using the numbering given in TrEMBL entry Q7Z314 (GeneID 55656).
Database link: Q75QN2 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18050の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/5000. Detects a band of approximately 120 kDa.
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| 特記事項 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/5000. Detects a band of approximately 120 kDa. |
ターゲット情報
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関連性
FLJ20530 is a component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3' box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 55656 Human
- Entrez Gene: 72656 Mouse
- Omim: 611351 Human
- SwissProt: Q75QN2 Human
- SwissProt: Q80V86 Mouse
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別名
- C8orf52 antibody
- Chromosome 8 open reading frame 52 antibody
- FLJ20530 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLJ20530 antibody (ab18050)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FLJ20530 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-FLJ20530 antibody (ab18050)Western blot using ab18050 at 0.2µ g/ml on HeLa nuclear lysate.
Lysate: 5µ g/lane.
Detection: Chemiluminescence with an exposure time of 20 minutes. Western blot using ab18050 at 0.2µg/ml on HeLa nuclear lysate.Lysate: 5µg/lane.
Detection: Chemiluminescence with an exposure time of 20 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab18050 は 1 報の論文で使用されています。
- Zhou Q et al. INTS8 is a therapeutic target for intrahepatic cholangiocarcinoma via the integration of bioinformatics analysis and experimental validation. Sci Rep 11:23649 (2021). PubMed: 34880328