Anti-FLIP 抗体 (ab8421)
Key features and details
- Rabbit polyclonal to FLIP
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FLIP antibody
FLIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FLIP -
由来種
Rabbit -
特異性
Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta. -
アプリケーション
適用あり: WB, ICC/IF, IHC-P, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human FLIP aa 2-19.
Sequence:SAEVIHQVEEALDTDEKE
(Peptide available asab8457) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
FLIP Antibody is affinity chromatography purified via peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8421の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (10) |
1/500 - 1/1000. Predicted molecular weight: 55 kDa.
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| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| IHC-P |
Use a concentration of 5 µg/ml.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 55 kDa. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. -
組織特異性
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. -
配列類似性
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
ドメイン
The caspase domain lacks the active sites residues involved in catalysis. -
翻訳後修飾
Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. - Information by UniProt
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参照データベース
- Entrez Gene: 8837 Human
- Entrez Gene: 12633 Mouse
- Entrez Gene: 117279 Rat
- Omim: 603599 Human
- SwissProt: O15519 Human
- SwissProt: O35732 Mouse
- Unigene: 390736 Human
- Unigene: 336848 Mouse
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別名
- c FLIP antibody
- c FLIPL antibody
- c FLIPR antibody
see all
画像
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Western blot - Anti-FLIP antibody (ab8421)Lane 1 : Anti-FLIP antibody (ab8421) at 1 µg/ml
Lane 2 : Anti-FLIP antibody (ab8421) at 2 µg/ml
All lanes : K562 cell lysate
Predicted band size: 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (ab8421)Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab8421)ab8421 at 5µg/ml staining FLIP in Hela cells by ICC/IF
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab8421 は 24 報の論文で使用されています。
- Zhang D et al. cFLIPL alleviates myocardial ischemia-reperfusion injury by regulating pyroptosis. Cell Biol Int 48:60-75 (2024). PubMed: 37750485
- Wang Y et al. The gene RAD51AP1 promotes the progression of pancreatic cancer via the PI3K/Akt/NF-κB signaling pathway. Neoplasma 70:722-732 (2023). PubMed: 37962862
- Kamiya M et al. Targeting necroptosis in muscle fibers ameliorates inflammatory myopathies. Nat Commun 13:166 (2022). PubMed: 35013338
- Jian JM et al. Naturally-Occurring Antibodies Against Bim are Decreased in Alzheimer's Disease and Attenuate AD-type Pathology in a Mouse Model. Neurosci Bull 38:1025-1040 (2022). PubMed: 35570231
- Ke C et al. Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib. Cancers (Basel) 12:N/A (2020). PubMed: 32375399