AB8421

Anti-FLIP 抗体

Name: Anti-FLIP 抗体 (ab8421) | Abcam
Brand: Abcam Limited
SKU: AB8421
Price: 95000 JPY
Availability: InStock
Rating: 4 (11 reviews)

Anti-FLIP antibody

(11 Reviews)

(29 Publications)

Rabbit Polyclonal FLIP antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human CFLAR aa 1-50.

別名を表示する

CASH, CASP8AP1, CLARP, MRIT, CFLAR, CASP8 and FADD-like apoptosis regulator, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, Inhibitor of FLICE, MACH-related inducer of toxicity, Usurpin, Casper, c-FLIP, FLAME-1, I-FLICE

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (AB8421)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (AB8421)

ab8421 at 5μg/ml staining FLIP in Hela cells by ICC/IF

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (AB8421)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (AB8421)

Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 μg/ml.

Unknown

Western blot - Anti-FLIP antibody (AB8421)

Lane 1:

Western blot - Anti-FLIP antibody (ab8421) at 1 µg/mL

Lane 2:

Western blot - Anti-FLIP antibody (ab8421) at 2 µg/mL

All lanes:

K562 cell lysate

Predicted band size: 55 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

軽鎖のタイプ

unknown

キャリアフリー

交差種

Mouse, Human, Rat

アプリケーション

WB, Flow Cyt, ICC/IF, IHC-P

applications

免疫原

Synthetic Peptide within Human CFLAR aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O15519

特異性

Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

FLIP Antibody is affinity chromatography purified via peptide column.

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FLIP also known as c-FLIP or CASP8 and FADD-like apoptosis regulator is an important protein that inhibits apoptosis. It has a molecular mass of around 55-58 kDa and comes in multiple isoforms including c-FLIP(L) and c-FLIP(S). FLIP is expressed in many tissues such as the thymus spleen and lungs and plays a role in immune regulation by modulating apoptosis induced by death receptors like Fas and TNFR1. Its regulation of cell death makes it key to cell survival especially in the context of immune cells.

Biological function summary

FLIP protein impacts cellular processes by blocking the activation of caspase-8 preventing the apoptosis cascade from proceeding. It is not typically part of a large complex but interacts directly with the death-inducing signaling complex (DISC). By inhibiting the apoptotic machinery it protects cells from unnecessary death signals facilitating controlled cellular turnover and immune responses.

Pathways

FLIP affects apoptosis and NF-kB signaling. These pathways are vital for cell survival inflammation and immune responses. FLIP interacts closely with Fas-associated protein with death domain (FADD) and caspase-8 to regulate apoptotic pathways. It acts as a checkpoint in intrinsic and extrinsic apoptosis pathways thereby influencing both normal and pathological processes such as immune cell survival and proliferation.

FLIP expression connects to cancer and autoimmune disorders. Its overexpression can contribute to cancer progression by allowing cancer cells to escape apoptosis leading to uncontrolled cell growth. Furthermore it can be linked to autoimmune disorders where inappropriate control of apoptosis results in prolonged survival of autoreactive immune cells. In these contexts FLIP’s interaction with proteins such as Fas and TNFR1 becomes significant highlighting its potential as a therapeutic target in mitigating these diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells (PubMed : 31046799). Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

See full target information CFLAR

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文献 (29)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 56:1013-1026 PubMed38684915

2024

Glutamine-mediated epigenetic regulation of cFLIP underlies resistance to TRAIL in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hye Kim,Jinyoung Lee,Se Seul Im,Boyun Kim,Eun-Young Kim,Hyo-Jin Min,Jinbeom Heo,Eun-Ju Chang,Kyung-Chul Choi,Dong-Myung Shin,Jaekyoung Son

Neoplasma 70:722-732 PubMed37962862

2023

The gene RAD51AP1 promotes the progression of pancreatic cancer via the PI3K/Akt/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongkun Wang,Yingchun Li,Cui Ran,Wenjun Le,Jiaxing Dong,Xujing Wang,Bo Chen,Xiaohua Jiang

Cell biology international 48:60-75 PubMed37750485

2023

cFLIP alleviates myocardial ischemia-reperfusion injury by regulating pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhang,Hui Wu,Di Liu,Ming Ye,Yunzhao Li,Gang Zhou,QingZhuo Yang,YanFang Liu,Yi Li

Fundamental research 5:296-306 PubMed40166102

2022

Epigenetics disruptions enabled by porphyrin-derived metal-organic frameworks disarm resistances to sonocatalytic ROS anti-tumor actions.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Dou Du,Chao Fang,Xin Yu,Yujia Fang,Xinyu Liu,Di Ou,Haohao Yin,Hui Liu,Taixia Wang,Lu Lu,Xiaolong Li,Kun Zhang

Cell death & disease 13:522 PubMed35661704

2022

Decoy receptor 2 mediates the apoptosis-resistant phenotype of senescent renal tubular cells and accelerates renal fibrosis in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Chen,Ke-Hong Chen,Li-Ming Wang,Jia Luo,Quan-You Zheng,Ya-Ni He

Neuroscience bulletin 38:1025-1040 PubMed35570231

2022

Naturally-Occurring Antibodies Against Bim are Decreased in Alzheimer's Disease and Attenuate AD-type Pathology in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Ming Jian,Dong-Yu Fan,Ding-Yuan Tian,Yuan Cheng,Pu-Yang Sun,Cheng-Rong Tan,Gui-Hua Zeng,Chen-Yang He,Ye-Ran Wang,Jie Zhu,Xiu-Qing Yao,Yan-Jiang Wang,Yu-Hui Liu

Nature communications 13:166 PubMed35013338

2022

Targeting necroptosis in muscle fibers ameliorates inflammatory myopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Mari Kamiya,Fumitaka Mizoguchi,Kimito Kawahata,Dengli Wang,Masahiro Nishibori,Jessica Day,Cynthia Louis,Ian P Wicks,Hitoshi Kohsaka,Shinsuke Yasuda

Oncology reports 45:230-238 PubMed33416145

2021

Targeting eIF4A using rocaglate CR‑1‑31B sensitizes gallbladder cancer cells to TRAIL‑mediated apoptosis through the translational downregulation of c‑FLIP.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Cao,Ying He,Litao Yang,Zhou Luan

Diagnostics (Basel, Switzerland) 10: PubMed33198065

2020

Hypo-Expression of Flice-Inhibitory Protein and Activation of the Caspase-8 Apoptotic Pathways in the Death-Inducing Signaling Complex Due to Ischemia Induced by the Compression of the Asphyxiogenic Tool on the Skin in Hanging Cases.

Applications

Unspecified application

Species

Unspecified reactive species

Aniello Maiese,Alessandra De Matteis,Giorgio Bolino,Emanuela Turillazzi,Paola Frati,Vittorio Fineschi

Cancers 12: PubMed32375399

2020

Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib.

Applications

Unspecified application

Species

Unspecified reactive species

Changhong Ke,Huan Hou,Jiayu Li,Kui Su,Chaohong Huang,Yue Lin,Zhiqiang Lu,Zhiyun Du,Wen Tan,Zhengqiang Yuan

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Anti-FLIP 抗体

Anti-FLIP antibody

Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (AB8421)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (AB8421)

Western blot - Anti-FLIP antibody (AB8421)

Key facts

宿主種

クローン性

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (29)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise