Anti-FLAP 抗体 (ab53536)
Key features and details
- Goat polyclonal to FLAP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FLAP antibody
FLAP 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to FLAP -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Cynomolgus monkey, Rhesus monkey
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免疫原
Synthetic peptide:
C-HKVEHESRTQNGR
, corresponding to internal sequence amino acids 28-40 of Human FLAP -
ポジティブ･コントロール
- WB: Human U937 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53536の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. -
関連疾患
Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. -
配列類似性
Belongs to the MAPEG family. -
ドメイン
The C-terminal part after residue 140 is mostly unstructured. -
細胞内局在
Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 102132235 Cynomolgus monkey
- Entrez Gene: 100034178 Horse
- Entrez Gene: 241 Human
- Entrez Gene: 100009413 Rabbit
- Entrez Gene: 713170 Rhesus monkey
- Omim: 603700 Human
- SwissProt: Q2PG08 Cynomolgus monkey
- SwissProt: P30353 Horse
see all -
別名
- 5-lipoxygenase activating protein antibody
- AL5AP_HUMAN antibody
- ALOX 5AP antibody
see all
画像
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Western blot - Anti-FLAP antibody (ab53536)Anti-FLAP antibody (ab53536) at 0.1 µg/ml + Human U937 cell lysate (35µg protein in RIPA buffer).
Predicted band size: 18 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab53536 は 3 報の論文で使用されています。
- Weisser H et al. Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function. Cancer Gene Ther 30:108-123 (2023). PubMed: 36114329
- Göbel T et al. Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancer. Life Sci Alliance 6:N/A (2023). PubMed: 36849252
- Hao Y et al. Transcriptomic Signatures and Functional Network Analysis of Chronic Rhinosinusitis With Nasal Polyps. Front Genet 12:609754 (2021). PubMed: 33603773