Anti-FKBP52 抗体 [EPR6619] (ab124906)
![Western blot - Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.co.jp/ps/products/124/ab124906/Images/ab124906-138889-anti-fkbp52-antibody-epr6619-western-blot.jpg "Western blot - Anti-FKBP52 antibody [EPR6619] (ab124906)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6619] to FKBP52
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FKBP52 antibody [EPR6619]
FKBP52 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6619] to FKBP52 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt (Intra) or ICC/IF -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide within Human FKBP52 aa 400-500 (C terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- HeLa, MCF7, Jurkat and K562 cell lysates; Human breast tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数（KD 値）
KD = 1.38 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6619 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124906の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/50000 - 1/200000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/50000 - 1/200000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
追加情報
Is unsuitable for Flow Cyt (Intra) or ICC/IF.
ターゲット情報
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機能
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. -
組織特異性
Widely expressed. -
配列類似性
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
ドメイン
The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400. -
翻訳後修飾
Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 2288 Human
- Omim: 600611 Human
- SwissProt: Q02790 Human
- Unigene: 524183 Human
- Unigene: 713721 Human
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別名
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
see all
画像
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Western blot - Anti-FKBP52 antibody [EPR6619] (ab124906)All lanes : Anti-FKBP52 antibody [EPR6619] (ab124906) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.co.jp/ps/products/124/ab124906/Images/ab124906-138888-anti-fkbp52-antibody-epr6619-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6619] (ab124906)ab124906, at 1/100 dilution, staining FKBP52 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.co.jp/ps/products/124/ab124906/Images/ab124906-138887-anti-fkbp52-antibody-epr6619.jpg)
OI-RD Scanning - Anti-FKBP52 antibody [EPR6619] (ab124906) -
![Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.co.jp/ps/products/124/ab124906/Images/ab124906-4-benefits-of-recombinant-antibodies.png)
Anti-FKBP52 antibody [EPR6619] (ab124906)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab124906 は 1 報の論文で使用されています。
- Maeda K et al. FKBP51 and FKBP52 regulate androgen receptor dimerization and proliferation in prostate cancer cells. Mol Oncol 16:940-956 (2022). PubMed: 34057812