Anti-FKBP52 抗体 [EPR6618] (ab129097)
![Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.co.jp/ps/products/129/ab129097/Images/ab129097-143074-anti-fkbp52-antibody-epr6618-western-blot.jpg "Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6618] to FKBP52
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FKBP52 antibody [EPR6618]
FKBP52 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6618] to FKBP52 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human FKBP52 aa 1-100. The exact sequence is proprietary.
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ポジティブ･コントロール
- HeLa, Jurkat, K562, and MCF7 cell lysates; Human colon and kidney tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解離定数（KD 値）
KD = 9.40 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR6618 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129097の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. -
組織特異性
Widely expressed. -
配列類似性
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
ドメイン
The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400. -
翻訳後修飾
Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 2288 Human
- Entrez Gene: 14228 Mouse
- Entrez Gene: 260321 Rat
- Omim: 600611 Human
- SwissProt: Q02790 Human
- SwissProt: P30416 Mouse
- SwissProt: Q9QVC8 Rat
- Unigene: 524183 Human
see all -
別名
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
see all
画像
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Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)All lanes : Anti-FKBP52 antibody [EPR6618] (ab129097) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : K562 cell lysates
Lane 4 : MCF7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.co.jp/ps/products/129/ab129097/Images/ab129097-263733-anti-fkbp52-antibody-epr6618-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (ab129097)ab129097 staining FKBP52 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.co.jp/ps/products/129/ab129097/Images/ab129097-143073-anti-fkbp52-antibody-epr6618-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.co.jp/ps/products/129/ab129097/Images/ab129097-143072-anti-fkbp52-antibody-epr6618-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.co.jp/ps/products/129/ab129097/Images/ab129097-143071-anti-fkbp52-antibody-epr6618.jpg)
OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (ab129097) -
![Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.co.jp/ps/products/129/ab129097/Images/ab129097-6-benefits-of-recombinant-antibodies.png)
Anti-FKBP52 antibody [EPR6618] (ab129097)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab129097 は 11 報の論文で使用されています。
- Han PZ et al. Development and validation of a gene model predicting lymph node metastasis and prognosis of oral squamous cell carcinoma based on single-cell and bulk RNA-seq analysis. J Oral Pathol Med 52:389-401 (2023). PubMed: 36153671
- Varastehmoradi B et al. Kappa opioid activation changes protein profiles in different regions of the brain relevant to depression. Eur Neuropsychopharmacol 72:9-17 (2023). PubMed: 37040689
- Lin CC et al. Effects of RU486 in Treatment of Traumatic Stress-Induced Glucocorticoid Dysregulation and Fear-Related Abnormalities: Early versus Late Intervention. Int J Mol Sci 23:N/A (2022). PubMed: 35628305
- Zong S et al. FKBP4 integrates FKBP4/Hsp90/IKK with FKBP4/Hsp70/RelA complex to promote lung adenocarcinoma progression via IKK/NF-κB signaling. Cell Death Dis 12:602 (2021). PubMed: 34112753
- Shao J et al. Hyperglycemia-induced increasing of RELB/circ_0008590 in NF-κB pathway is repressed by miR-1243 in human retinal microvascular endothelial cells. Ann Transl Med 9:1624 (2021). PubMed: 34926668