Anti-FKBP52 抗体 [EPR21125] (ab230951)
![Western blot - Anti-FKBP52 antibody [EPR21125] (ab230951)](https://www.abcam.co.jp/ps/products/230/ab230951/Images/ab230951-1-230951WB1.jpg "Western blot - Anti-FKBP52 antibody [EPR21125] (ab230951)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21125] to FKBP52
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FKBP52 antibody [EPR21125]
FKBP52 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR21125] to FKBP52 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: K562, HeLa and HEK-293T cell lysate; Human fetal brain lysate. ICC/IF: HeLa and MCF7 cells. Flow Cyt (intra): HeLa cells. IP: HeLa cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21125 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab230951の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
|
| WB |
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
|
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| ICC/IF |
1/100.
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| IP |
1/30.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
|
ICC/IF
1/100. |
|
IP
1/30. |
ターゲット情報
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機能
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. -
組織特異性
Widely expressed. -
配列類似性
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
ドメイン
The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400. -
翻訳後修飾
Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 2288 Human
- Omim: 600611 Human
- SwissProt: Q02790 Human
- Unigene: 524183 Human
- Unigene: 713721 Human
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別名
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
see all
画像
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Western blot - Anti-FKBP52 antibody [EPR21125] (ab230951)Lanes 1-2 : Anti-FKBP52 antibody [EPR21125] (ab230951) at 1/5000 dilution
Lanes 3-4 : Anti-FKBP52 antibody [EPR21125] (ab230951) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 4 : Human fetal brain lysate at 10 µg
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking/Diluting buffer: 5% NFDM/TBST.
Exposure times: Lane 1-2: 48 seconds; Lane 3: 30 seconds; Lane 4: 3 minutes.
The molecular mass observed is consistent with what has been described in the literature (PMID: 26065228).
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![Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR21125] (ab230951)](https://www.abcam.co.jp/ps/products/230/ab230951/Images/ab230951-2-230951ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR21125] (ab230951)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling FKBP52 with ab230951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HeLa cell line.
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of the primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunoprecipitation - Anti-FKBP52 antibody [EPR21125] (ab230951)](https://www.abcam.co.jp/ps/products/230/ab230951/Images/ab230951-5-230951IP1.jpg)
Immunoprecipitation - Anti-FKBP52 antibody [EPR21125] (ab230951)FKBP52 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab230951 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230951 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab230951 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230951 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR21125] (ab230951)](https://www.abcam.co.jp/ps/products/230/ab230951/Images/ab230951-3-230951ICC2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR21125] (ab230951)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling FKBP52 with ab230951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in MCF7 cell line.
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of the primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-FKBP52 antibody [EPR21125] (ab230951)](https://www.abcam.co.jp/ps/products/230/ab230951/Images/ab230951-4-230951FC1.jpg)
Flow Cytometry (Intracellular) - Anti-FKBP52 antibody [EPR21125] (ab230951)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling FKBP52 with ab230951 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor� 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Anti-FKBP52 antibody [EPR21125] (ab230951)](https://www.abcam.co.jp/ps/products/230/ab230951/Images/ab230951-6-benefits-of-recombinant-antibodies.png)
Anti-FKBP52 antibody [EPR21125] (ab230951)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab230951 は 1 報の論文で使用されています。
- Zong S et al. FKBP4 integrates FKBP4/Hsp90/IKK with FKBP4/Hsp70/RelA complex to promote lung adenocarcinoma progression via IKK/NF-κB signaling. Cell Death Dis 12:602 (2021). PubMed: 34112753