Anti-FKBP52 抗体 [EPR21120]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR21120] (AB230952)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling FKBP52 with ab230952 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in human breast cancer (PMID : 19584157) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR21120] (AB230952)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling FKBP52 with ab230952 at 1/1,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in rat cerebrum (PMID : 20133804; PMID : 20422297) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR21120] (AB230952)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling FKBP52 with ab230952 at 1/1,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in mouse cerebrum (PMID : 20133804; PMID : 20422297) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• WB

Lab

Western blot - Anti-FKBP52 antibody [EPR21120] (AB230952)

Western blot : Anti-FKBP52 antibody [EPR21120] ab230952 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 52 kDa in Wild-type HCT 116 cell lysates with no signal observed at this size in FKBP4 knockout HCT 116 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FKBP52 antibody [EPR21120] (ab230952) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 at 20 µg

Lane 2:

Western blot - Human FKBP4 knockout HCT116 cell line (ab300946) at 20 µg

Lane 3:

LNCaP at 20 µg

Lane 4:

THP-1 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

• WB

Supplier Data

Western blot - Anti-FKBP52 antibody [EPR21120] (AB230952)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-3 : 81 seconds; Lane 4 : 30 second; Lanes 5 : 120 seconds; Lane 6-8 : 3 minutes.

The molecular mass observed is consistent with what has been described in the literature (PMID : 26065228).

Lanes 1, 2, 3 and 7:

Western blot - Anti-FKBP52 antibody [EPR21120] (ab230952) at 1/5000 dilution

Lanes 4, 5, 6 and 8:

Western blot - Anti-FKBP52 antibody [EPR21120] (ab230952) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 5:

Mouse brain lysate at 10 µg

Lane 6:

Rat brain lysate at 10 µg

Lane 7:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 8:

Human fetal kidney lysate at 10 µg

Secondary

Lanes 1 - 7:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 8:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 52 kDa

false