Anti-FKBP25 抗体 (ab16654)
Key features and details
- Rabbit polyclonal to FKBP25
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FKBP25 antibody -
製品の詳細
Rabbit polyclonal to FKBP25 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Hamster, Cow, Monkey, African green monkey
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FKBP25.
Immunogen の所有権に関して (Peptide available as ab16979.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16654の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
Use a concentration of 0.05 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 0.05 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa). |
ターゲット情報
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機能
FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins. -
配列類似性
Belongs to the FKBP-type PPIase family.
Contains 1 PPIase FKBP-type domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 515069 Cow
- Entrez Gene: 2287 Human
- Entrez Gene: 30795 Mouse
- Entrez Gene: 100009004 Rabbit
- Entrez Gene: 299104 Rat
- Omim: 186947 Human
- SwissProt: P26884 Cow
- SwissProt: Q00688 Human
see all -
別名
- 25 kDa FK506-binding protein antibody
- 25 kDa FKBP antibody
- FK506 binding protein 25 T cell antibody
see all
画像
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Western blot - Anti-FKBP25 antibody (ab16654)All lanes : Anti-FKBP25 antibody (ab16654) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Lysate
Lane 3 : HeLa Whole Cell Lysate with Human FKBP25 peptide (ab16979) at 1 µg/ml
Lane 4 : HeLa Nuclear Lysate with Human FKBP25 peptide (ab16979) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
Western blot - Anti-FKBP25 antibody (ab16654)This image is courtesy of collaborator dataAll lanes : Anti-FKBP25 antibody (ab16654) at 0.05 µg/ml
Lane 1 : HeLa nuclear extracts
Lane 2 : HeLa nuclear extracts with Human FKBP25 peptide (ab16979) at 1 µg/ml
Lane 3 : HeLa nuclear extracts with Human FKBP25 peptide (ab16980) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDa
ab16654 specifically recognises FKBP25 (lane1), which can be blocked using the N-terminal immunizing peptide (lane2) but not the corresponding C-terminal peptide (lane3). -
Western blot - Anti-FKBP25 antibody (ab16654)All lanes : Anti-FKBP25 antibody (ab16654) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab16654 は 7 報の論文で使用されています。
- Wang D et al. FKBP25 Regulates Meiotic Apparatus During Mouse Oocyte Maturation. Front Cell Dev Biol 9:625805 (2021). PubMed: 33553183
- Yang Y et al. Esterase D stabilizes FKBP25 to suppress mTORC1. Cell Mol Biol Lett 26:50 (2021). PubMed: 34875997
- Tong J et al. FKBP3 mediates oxaliplatin resistance in colorectal cancer cells by regulating HDAC2 expression. Oncol Rep N/A:N/A (2019). PubMed: 31524278
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Zhu W et al. FKBP3 Promotes Proliferation of Non-Small Cell Lung Cancer Cells through Regulating Sp1/HDAC2/p27. Theranostics 7:3078-3089 (2017). PubMed: 28839465
- Schreiber KH et al. Rapamycin-mediated mTORC2 inhibition is determined by the relative expression of FK506-binding proteins. Aging Cell 14:265-73 (2015). WB . PubMed: 25652038
- Ochocka AM et al. FKBP25, a novel regulator of the p53 pathway, induces the degradation of MDM2 and activation of p53. FEBS Lett 583:621-6 (2009). WB ; Human . PubMed: 19166840