AB14432

Anti-FKBP15 抗体

Name: Anti-FKBP15 抗体 (ab14432) | Abcam
Brand: Abcam Limited
SKU: AB14432
Rating: 5 (4 reviews)

Anti-FKBP15 antibody

(4 Reviews)

(4 Publications)

Rabbit Polyclonal FKBP15 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human FKBP15 aa 50-100.

別名を表示する

KIAA0674, FKBP15, FK506-binding protein 15, FKBP-15, 133 kDa FK506-binding protein, WASP- and FKBP-like protein, 133 kDa FKBP, FKBP-133, WAFL

3 Images

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Western blot - Anti-FKBP15 antibody (AB14432)

In figure B the Immunoprecipitation was done using ab14432 (Lane 3), ab14433 (Lane 4) and ab14434 (Lane 5) at 2μg/mg of extract.

All lanes:

Western blot - Anti-FKBP15 antibody (ab14432) at 0.2 µg/mL

Lane 1:

Nuclear lysates prepared from Hela cells at 5 µg

Lane 2:

Nuclear lysates prepared from Hela cells at 20 µg

Lanes 3 - 5:

Nuclear lysates prepared from Hela cells at 1 mg/mL

Predicted band size: 135 kDa

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Supplier Data

Western blot - Anti-FKBP15 antibody (AB14432)

All lanes:

Western blot - Anti-FKBP15 antibody (ab14432) at 0.1 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 134 kDa

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Exposure time: 3min

Unknown

Western blot - Anti-FKBP15 antibody (AB14432)

Antibodies : ab14432 used at 0.2 mcg/ml for WB in A and B and 2 mcg/mg extract for IP (B), ab14433 and ab14434 used at 2 mcg/mg extract for IP. Detection : Chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B). In figure B the Immunoprecipitation was done using ab14432 (Lane 3), ab14433 (Lane 4) and ab14434 (Lane 5) at 2µg/mg of extract.

All lanes:

Western blot - Anti-FKBP15 antibody (ab14432) at 0.2 µg/mL

Lane 1:

Nuclear lysates prepared from Hela cells at 5 µg

Lane 2:

Nuclear lysates prepared from Hela cells at 20 µg

Lanes 3 - 5:

Nuclear lysates prepared from Hela cells at 1 mg/mL

Predicted band size: 134 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human FKBP15 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q5T1M5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Antibody was purified using an epitope specific to FKBP-135 immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FKBP15 also known as FK506-binding protein 15 is a protein that functions as a peptidyl-prolyl cis-trans isomerase facilitating protein folding. It possesses a molecular mass of approximately 20 kDa. This protein is expressed mainly in tissues such as the brain and testis but can also be found in other tissues at lower levels. The primary role of FKBP15 involves protein folding and stabilization which is important for maintaining cellular functions.

Biological function summary

FK506-binding protein 15 assists in modulating protein-protein interactions due to its isomerase activity. FKBP15 is involved in the secretory pathway and may associate with various intracellular complexes to exert its function. This association enhances its ability to regulate proteins trafficking and processing within the endoplasmic reticulum ensuring proteins reach their correct destinations within the cell.

Pathways

FKBP15 plays a role in intracellular signaling and immune regulation. It participates in the calcineurin signaling pathway where it regulates calcium ion-dependent processes linking it to proteins such as calcineurin and NF-AT. FKBP15 also engages with the endoplasmic reticulum-associated degradation (ERAD) pathway contributing to the degradation of misfolded proteins and maintaining cellular homeostasis.

FKBP15 has links to neurodegenerative disorders and cancer. In neurodegeneration disrupted FKBP15 function can affect protein folding balance connecting to proteins like tau which is implicated in Alzheimer's disease. In cancer FKBP15's role in protein folding and signaling pathways may influence cellular proliferation and tumor development providing a potential therapeutic target for drug development.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May be involved in the cytoskeletal organization of neuronal growth cones. Seems to be inactive as a PPIase (By similarity). Involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement.

See full target information FKBP15

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed38003310

2023

MA Demethylase Inhibits Osteogenesis of Dental Follicle Stem Cells via Regulating miR-7974/FKBP15 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Linwei Zheng,Zhizheng Li,Bing Wang,Rui Sun,Yuqi Sun,Jiangang Ren,Jihong Zhao

Pigment cell & melanoma research 29:559-71 PubMed27390154

2016

Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin J Tyrrell,Emma F Woodham,Heather J Spence,Douglas Strathdee,Robert H Insall,Laura M Machesky

Nature communications 5:3828 PubMed24819384

2014

Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy.

Applications

ICC/IF

Species

Human

Eszter Zavodszky,Matthew N J Seaman,Kevin Moreau,Maria Jimenez-Sanchez,Sophia Y Breusegem,Michael E Harbour,David C Rubinsztein

International journal of colorectal disease 23:921-30 PubMed18654788

2008

Identification of a new WASP and FKBP-like (WAFL) protein in inflammatory bowel disease: a potential marker gene for ulcerative colitis.

Applications

IHC (PFA fixed)

Species

Human

Ing-Marie Viklund,Nikolai V Kuznetsov,Robert Löfberg,Marco Daperno,Raffaello Sostegni,Marco Astegiano,Mario Rizzetto,Oliver von Stein,Mauro D'Amato,Petra von Stein,Sven Pettersson

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FKBP15 抗体

Anti-FKBP15 antibody

Western blot - Anti-FKBP15 antibody (AB14432)

Western blot - Anti-FKBP15 antibody (AB14432)

Western blot - Anti-FKBP15 antibody (AB14432)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise