Anti-FKBP12 抗体 [EP3887] (ab108420)
![Western blot - Anti-FKBP12 antibody [EP3887] (ab108420)](https://www.abcam.co.jp/ps/products/108/ab108420/Images/ab108420-297790-anti-fkbp12-antibody-ep3887-western-blot.jpg "Western blot - Anti-FKBP12 antibody [EP3887] (ab108420)")
Key features and details
- Rabbit monoclonal [EP3887] to FKBP12
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-FKBP12 antibody [EP3887]
FKBP12 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP3887] to FKBP12 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Fetal brain, HL-60, U937 and SH-SY5Y lysates; ICC: HeLa cells
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP3887 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108420の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
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| ICC/IF |
1/50 - 1/100.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 12 kDa. |
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ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
配列類似性
Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
Contains 1 PPIase FKBP-type domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2280 Human
- Entrez Gene: 14225 Mouse
- Entrez Gene: 25639 Rat
- Omim: 186945 Human
- SwissProt: P62942 Human
- SwissProt: P26883 Mouse
- SwissProt: Q62658 Rat
- Unigene: 471933 Human
see all -
別名
- 12 kDa FK506-binding protein antibody
- 12 kDa FKBP antibody
- Calstabin 1 antibody
see all
画像
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Western blot - Anti-FKBP12 antibody [EP3887] (ab108420)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab108420 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108420 was shown to recognize FKBP12 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP12 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab108420 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-FKBP12 antibody [EP3887] (ab108420)](https://www.abcam.co.jp/ps/products/108/ab108420/Images/ab108420-121137-anti-fkbp12-antibody-ep3887-western-blot.jpg)
Western blot - Anti-FKBP12 antibody [EP3887] (ab108420)All lanes : Anti-FKBP12 antibody [EP3887] (ab108420) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
Lane 3 : U937 lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 12 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody [EP3887] (ab108420)](https://www.abcam.co.jp/ps/products/108/ab108420/Images/ab108420-121136-anti-fkbp12-antibody-ep3887-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody [EP3887] (ab108420)Immunofluorescent staining of FKBP12 in HeLa cells using ab108420 at 1/50 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab108420 は 3 報の論文で使用されています。
- Marcadet L et al. RANKL Inhibition Reduces Cardiac Hypertrophy in mdx Mice and Possibly in Children with Duchenne Muscular Dystrophy. Cells 12:N/A (2023). PubMed: 37296659
- Hu H et al. RIP3-mediated necroptosis is regulated by inter-filament assembly of RIP homotypic interaction motif. Cell Death Differ 28:251-266 (2021). PubMed: 32737444
- Hu H et al. ICP6 Prevents RIP1 Activation to Hinder Necroptosis Signaling. Front Cell Dev Biol 8:595253 (2020). PubMed: 33195272