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AB24373

Anti-FKBP12 抗体

Anti-FKBP12 antibody

5

(4 Reviews)

|

(18 Publications)

Anti-FKBP12 antibody (ab24373) is a rabbit polyclonal antibody detecting FKBP12 in Western Blot. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Over 10 publications
- Trusted since 2006

別名を表示する

FKBP1, FKBP12, FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, PPIase FKBP1A, 12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, Immunophilin FKBP12, Rotamase, 12 kDa FKBP, FKBP-12, FKBP-1A

3 Images
Western blot - Anti-FKBP12 antibody (AB24373)
  • WB

Lab

Western blot - Anti-FKBP12 antibody (AB24373)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : FKBP1A knockout HAP1 whole cell lysate (20 μg)
Lane 3 : SH-SY5Y whole cell lysate (20 μg)
Lane 4 : Human brain whole tissue lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab24373 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab24373 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP1A knockout cells. Wild-type and FKBP1A knockout samples were subjected to SDS-PAGE. ab24373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FKBP12 antibody (ab24373)

Predicted band size: 12 kDa

false

Western blot - Anti-FKBP12 antibody (AB24373)
  • WB

Ap

Western blot - Anti-FKBP12 antibody (AB24373)

All lanes:

Western blot - Anti-FKBP12 antibody (ab24373) at 1 µg/mL

Lane 1:

Human Brain Tissue Lysate at 20 µg

Lane 2:

Mouse Brain Tissue Lysate at 20 µg

Lane 3:

Rat Brain Tissue Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

Western blot - Anti-FKBP12 antibody (AB24373)
  • WB

Unknown

Western blot - Anti-FKBP12 antibody (AB24373)

All lanes:

Western blot - Anti-FKBP12 antibody (ab24373) at 1 µg/mL

All lanes:

Recombinant Human FKBP12 protein (ab56524) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 12 kDa

true

Exposure time: 2min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

What is this antibody validated in?
Anti-FKBP12 antibody (ab24373) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of FKBP12?
Anti-FKBP12 (ab24373) specifically detects a band for FKBP12 (UniProt: P62942) at a molecular weight of 12kDa.

Trusted by the scientific community
Anti-FKBP12 (ab24373) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.

Specificity confirmed
The specificity of Anti-FKBP12 antibody (ab24373) has been confirmed by Western blot testing in FKBP1A Knockout HAP1 cells.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FKBP12 also known as FKBP-12 or FKBP12 protein is a small protein with a molecular weight of approximately 12 kDa. It belongs to the family of immunophilins and acts as a peptidyl-prolyl cis-trans isomerase (PPIase) facilitating the folding of proteins by catalyzing the isomerization of proline residues in polypeptides. The FKBP12 protein is expressed in various tissues throughout the body including the brain heart and skeletal muscles. It plays a mechanical role in binding certain macrolide antibiotics such as FK506 (tacrolimus) influencing their biological effects by altering protein conformation.
Biological function summary

FKBP12 is significant in cellular mechanisms beyond protein folding. It interacts with and forms complexes with other proteins such as ryanodine receptors and transforming growth factor-beta (TGF-Β) receptors impacting calcium signaling and cell growth. FKBP12's interaction with FK506 has been extensively studied because it forms a complex that inhibits calcineurin ultimately leading to immunosuppressive effects. This property of FKBP12 makes it important for modulating the immune response and has importance in transplant medicine.

Pathways

FKBP12 is actively involved in calcium signaling and TGF-Β signaling pathways. FKBP12 influences these pathways by binding to ryanodine receptors affecting calcium release from intracellular stores and also modulating the function of TGF-Β receptors impacting cellular growth and proliferation processes. It is associated with proteins such as calcineurin and Smad proteins in these pathways maintaining cellular homeostasis and regulating immune and inflammatory responses.

FKBP12 plays a role in immunological and proliferative diseases. It is implicated in autoimmune disorders due to its interaction with FK506 leading to the suppression of unwanted immune responses. FKBP12 is also linked to cardiac hypertrophy as its modulation of calcium release through ryanodine receptors can affect heart muscle function. The protein interacts with calcineurin in these contexts contributing to pathological processes in these diseases. Researchers target FKBP12 with ELISA and other assays to investigate its involvement in these disorders and assess therapeutic interventions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand. Recruits SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. May modulate the RYR1 calcium channel activity. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
See full target information FKBP1A
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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110423 PubMed39104417

2024

Targeted dephosphorylation of SMAD3 as an approach to impede TGF-β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Abigail Brewer,Jin-Feng Zhao,Rotimi Fasimoye,Natalia Shpiro,Thomas J Macartney,Nicola T Wood,Melanie Wightman,Dario R Alessi,Gopal P Sapkota

Molecular cell 84:2436-2454.e10 PubMed38925114

2024

pLxIS-containing domains are biochemically flexible regulators of interferons and metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren M Landau,Neha Chaudhary,Yun Chen Tien,Magdalena Rogozinska,Shakchhi Joshi,Conghui Yao,Joseph Crowley,Karthik Hullahalli,Ian W Campbell,Matthew K Waldor,Marcia Haigis,Jonathan C Kagan

The Journal of experimental medicine 221: PubMed38861480

2024

A viral E3 ubiquitin ligase produced by herpes simplex virus 1 inhibits the NLRP1 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Pooja Parameswaran,Laurellee Payne,Jennifer Powers,Mehdi Rashighi,Megan H Orzalli

iScience 27:109302 PubMed38450154

2024

Mapping the substrate landscape of protein phosphatase 2A catalytic subunit PPP2CA.

Applications

Unspecified application

Species

Unspecified reactive species

Abigail Brewer,Gajanan Sathe,Billie E Pflug,Rosemary G Clarke,Thomas J Macartney,Gopal P Sapkota

Cell chemical biology 31:1162-1175.e10 PubMed38320555

2024

The cyclimids: Degron-inspired cereblon binders for targeted protein degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Saki Ichikawa,N Connor Payne,Wenqing Xu,Chia-Fu Chang,Nandini Vallavoju,Spencer Frome,Hope A Flaxman,Ralph Mazitschek,Christina M Woo

Cell reports 42:113581 PubMed38103201

2023

The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick M Exconde,Claudia Hernandez-Chavez,Christopher M Bourne,Rachel M Richards,Mark B Bray,Jan L Lopez,Tamanna Srivastava,Marisa S Egan,Jenna Zhang,William Yoo,Sunny Shin,Bohdana M Discher,Cornelius Y Taabazuing

Communications biology 6:861 PubMed37596377

2023

Plasmodium falciparum formins are essential for invasion and sexual stage development.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Collier,Emma Pietsch,Madeline Dans,Dawson Ling,Tatyana A Tavella,Sash Lopaticki,Danushka S Marapana,Mohini A Shibu,Dean Andrew,Snigdha Tiash,Paul J McMillan,Paul Gilson,Leann Tilley,Matthew W A Dixon

iScience 25:105139 PubMed36193052

2022

Dissection of the MEF2D-IRF8 transcriptional circuit dependency in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Y Pingul,Hua Huang,Qingzhou Chen,Fatemeh Alikarami,Zhen Zhang,Jun Qi,Kathrin M Bernt,Shelley L Berger,Zhendong Cao,Junwei Shi

Cell chemical biology 29:1482-1504.e7 PubMed36075213

2022

Target protein localization and its impact on PROTAC-mediated degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Luke M Simpson,Lorraine Glennie,Abigail Brewer,Jin-Feng Zhao,Jennifer Crooks,Natalia Shpiro,Gopal P Sapkota

PLoS pathogens 18:e1009882 PubMed35930605

2022

Deletion of the Plasmodium falciparum exported protein PTP7 leads to Maurer's clefts vesiculation, host cell remodeling defects, and loss of surface presentation of EMP1.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia M S Carmo,Gerald J Shami,Dezerae Cox,Boyin Liu,Adam J Blanch,Snigdha Tiash,Leann Tilley,Matthew W A Dixon
View all publications
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代替製品

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Primary Antibodies

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組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

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