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AB24853

FITC Anti-VCAM1 抗体 [M/K-2]

FITC Anti-VCAM1 antibody [M/K-2]

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(7 Publications)

Rat Monoclonal VCAM1 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 7 publications.

別名を表示する

CD106, Vcam-1, Vcam1, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1

1 Images
Flow Cytometry - FITC Anti-VCAM1 antibody [M/K-2] (AB24853)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-VCAM1 antibody [M/K-2] (AB24853)

Flow Cytometry analysis of C57BL/6 mouse bone marrow cells stained with anti VCAM1 antibody [M/K-2] (FITC) (ab24853) and Rat Anti-Mouse Ly-6G/Ly-6C-PE. 1 μg of antibody was used for 106 cells.

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

M/K-2

アイソタイプ

IgG1

軽鎖のタイプ

kappa

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/fitc-rat-igg1-kappa-rtk2071-isotype-control-ab18404'>ab18404</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
バッファー組成
pH: 7.4
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.
See full target information Vcam1
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Neurobiology of disease 181:106125 PubMed37062307

2023

Amyloid beta peptides (Aβ) from Alzheimer's disease neuronal secretome induce endothelial activation in a human cerebral microvessel model.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Jung Shin,Kira M Evitts,Solhee Jin,Caitlin Howard,Margaret Sharp-Milgrom,Tiara Schwarze-Taufiq,Chizuru Kinoshita,Jessica E Young,Ying Zheng

Bone 123:234-245 PubMed30953717

2019

Vasculature submucosal changes at early stages of osteonecrosis of the jaw (ONJ).

Applications

Unspecified application

Species

Unspecified reactive species

Ioannis Gkouveris,Danny Hadaya,Akrivoula Soundia,Olga Bezouglaia,Yee Chau,Sarah M Dry,Flavia Q Pirih,Tara L Aghaloo,Sotirios Tetradis

Bioconjugate chemistry 27:1921-30 PubMed27315634

2016

Paramagnetic Phospholipid-Based Micelles Targeting VCAM-1 Receptors for MRI Visualization of Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Amerigo Pagoto,Rachele Stefania,Francesca Garello,Francesca Arena,Giuseppe Digilio,Silvio Aime,Enzo Terreno

Molecular medicine reports 14:1302-8 PubMed27279073

2016

Protective effects of BMSCs in combination with erythropoietin in bronchopulmonary dysplasia-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Hua Zhang,Yan-Yan Pan,Rui-Sheng Jing,Yun Luan,Luan Zhang,Chao Sun,Feng Kong,Kai-Lin Li,Yi-Biao Wang

Molecular medicine reports 12:2511-20 PubMed25936350

2015

Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Lin Zeng,Li-Hui Wang,Ping Li,Wei Wang,Jiong Yang

Nature 514:498-502 PubMed25341788

2014

Dendritic cells control fibroblastic reticular network tension and lymph node expansion.

Applications

Flow Cyt

Species

Unspecified reactive species

Sophie E Acton,Aaron J Farrugia,Jillian L Astarita,Diego Mourão-Sá,Robert P Jenkins,Emma Nye,Steven Hooper,Janneke van Blijswijk,Neil C Rogers,Kathryn J Snelgrove,Ian Rosewell,Luis F Moita,Gordon Stamp,Shannon J Turley,Erik Sahai,Caetano Reis e Sousa

Stem cells (Dayton, Ohio) 28:1465-75 PubMed20629173

2010

Major histocompatibility complex-I expression on embryonic stem cell-derived vascular progenitor cells is critical for syngeneic transplant survival.

Applications

Flow Cyt

Species

Mouse

Mingchao Ma,Shunli Ding,Andreas Lundqvist,Hong San,Fang Fang,Mikhail Konoplyannikov,Colin Berry,Leilani E Beltran,Guibin Chen,Jason C Kovacic,Manfred Boehm
View all publications
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代替製品

Primary Antibodies

AB223982

FITC Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - FITC Anti-VCAM1 antibody [EPR5047] (AB223982)

  • 0
  • 0
Primary Antibodies

AB195540

HRP Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-VCAM1 antibody [EPR5047] (AB195540)

  • 0
  • 0
Primary Antibodies

AB223981

PE Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-VCAM1 antibody [EPR5047] (AB223981)

  • 0
  • 0
Primary Antibodies

AB215380

Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free (AB215380)

  • 0
  • 0
Primary Antibodies

AB194319

Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047] (AB194319)

  • 5
  • 1
Primary Antibodies

AB174279

Anti-VCAM1 antibody [EPR5038(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

  • 0
  • 0
Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

  • 5
  • 16

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB226

FITC Anti-CD90 / Thy1 antibody [MRC OX-7]

Immunocytochemistry - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (AB226)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB282575

Anti-ICAM1 antibody [EPR24639-3]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR24639-3] (AB282575)

  • 5
  • 1

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