FITC Anti-Transferrin Receptor 抗体 [YTA 74.4] (ab33996)
Key features and details
- FITC Rat monoclonal [YTA 74.4] to Transferrin Receptor
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
製品の概要
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製品名
FITC Anti-Transferrin Receptor antibody [YTA 74.4]
Transferrin Receptor 一次抗体 製品一覧 -
製品の詳細
FITC Rat monoclonal [YTA 74.4] to Transferrin Receptor -
由来種
Rat -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Transferrin Receptor. Concanavilin A activated mouse spleen cells.
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特記事項
ab33996 stains dividing cells, and can be used to distinguish resting from activated T cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified IgG prepared by ion exchange chromatography. -
一次抗体 備考
ab33996 stains dividing cells, and can be used to distinguish resting from activated T cells. -
ポリ/モノ
モノクローナル -
クローン名
YTA 74.4 -
ミエローマ
Y3/Ag1.2.3 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33996の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
Use 10ul to label 10^6 cells in 100ul. ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. Use 10ul to label 10^6 cells in 100ul. ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
関連疾患
Immunodeficiency 46 -
配列類似性
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
翻訳後修飾
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
細胞内局在
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 22042 Mouse
- SwissProt: Q62351 Mouse
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別名
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab33996 は 4 報の論文で使用されています。
- Hauser D et al. Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing. Nanomaterials (Basel) 8:N/A (2018). PubMed: 30562983
- Fagoonee S et al. Lack of plasma protein hemopexin dampens mercury-induced autoimmune response in mice. J Immunol 181:1937-47 (2008). Flow Cyt ; Mouse . PubMed: 18641331
- Lesley J et al. Expression of transferrin receptor on murine hematopoietic progenitors. Cell Immunol 83:14-25 (1984). PubMed: 6319033
- Trowbridge IS et al. Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J Cell Physiol 112:403-10 (1982). PubMed: 6290505