FITC Anti-SIRP alpha 抗体 [03] (ab289909)
![Flow Cytometry - FITC Anti-SIRP alpha antibody [03] (ab289909)](https://www.abcam.co.jp/ps/products/289/ab289909/Images/ab289909-482658-fitcantisirpalphaantibody03flowcytometry.jpg "Flow Cytometry - FITC Anti-SIRP alpha antibody [03] (ab289909)")
Key features and details
- FITC Mouse monoclonal [03] to SIRP alpha
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
製品の概要
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製品名
FITC Anti-SIRP alpha antibody [03]
SIRP alpha 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [03] to SIRP alpha -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment (His-tag) corresponding to Human SIRP alpha aa 1-400 (extracellular). NP_542970.1. Polyhistidine tag at the C-terminus.
Database link: P78324 -
ポジティブ･コントロール
- Flow cyt: U937 cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 0.5% BSA, 99.41% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
03 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab289909の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
10 μl/Test |
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. 10 μl/Test |
ターゲット情報
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機能
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
組織特異性
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
配列類似性
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 140885 Human
- Omim: 602461 Human
- SwissProt: P78324 Human
- Unigene: 581021 Human
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別名
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
画像
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Flow Cytometry - FITC Anti-SIRP alpha antibody [03] (ab289909)
Flow cytometric analysis of U937 (human histiocytic lymphoma cell line) cells labeling SIRP alpha with ab275641 (Black) compared with an isotype control (Grey). Cells should be Fc-blocked by treatment with 20 μg of human IgG/106 cells for 1 hour at 4 prior to staining, washed, then stained with ab289909.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab289909 は論文での使用が確認できていません。