FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) 抗体 [MAC387] (ab7429)
![Flow Cytometry (Intracellular) - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab7429)](https://www.abcam.co.jp/ps/products/7/ab7429/Images/ab7429-12936-anti-s100a9-calprotectin-s100a8-a9-complex-antibody-mac387-fitc-flow-cytometry.jpg "Flow Cytometry (Intracellular) - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab7429)")
Key features and details
- FITC Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)
- Suitable for: Flow Cyt (Intra)
- Reacts with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig, Monkey, Baboon, Cynomolgus monkey, Rhesus monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]
S100A9 + Calprotectin (S100A8/A9 complex) 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex) -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
特異性
This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig, Monkey, Baboon, Cynomolgus monkey, Rhesus monkey -
免疫原
Tissue, cells or virus corresponding to Human S100A9 + Calprotectin (S100A8/A9 complex). Human monocytes
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ポジティブ･コントロール
- Flow Cyt (Intra): Human peripheral blood granulocyte
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
MAC387 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7429の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use 10µl for 106 cells.
Membrane permeabilisation is required for this application. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
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Flow Cyt (Intra)
Use 10µl for 106 cells. Membrane permeabilisation is required for this application. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
画像
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Flow Cytometry (Intracellular) - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab7429)
ab7429 staining S100A9 + Calprotectin (FITC) in human peripheral blood granulocyte by Intracellular Flow Cytometry analysis.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab7429 は 7 報の論文で使用されています。
- Monteiro LN et al. Tumour-associated macrophages: Relation with progression and invasiveness, and assessment of M1/M2 macrophages in canine mammary tumours. Vet J 234:119-125 (2018). IHC-P . PubMed: 29680383
- Burwitz BJ et al. Technical advance: liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease. J Leukoc Biol 96:491-501 (2014). PubMed: 24823811
- Gutierrez M et al. The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet Immunol Immunopathol 71:321-34 (1999). PubMed: 10587310
- Brandtzaeg P et al. The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathology 21:191-6 (1992). PubMed: 1505941
- Brandtzaeg P et al. Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen. J Clin Pathol 41:963-70 (1988). PubMed: 3056988
- Flavell DJ et al. Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J Histochem Cytochem 35:1217-26 (1987). PubMed: 3309045
- Taraballi F et al. Biomimetic collagenous scaffold to tune inflammation by targeting macrophages. J Tissue Eng 7:2041731415624667 (0). IHC . PubMed: 26977285