FITC Anti-RFP 抗体 [EPR28246-45]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-RFP antibody [EPR28246-45] (AB323681)

Flow cytometry staining of 293T cells transfected with a Red fluorescent protein expression vector containing a Myc-His-tag (upper right) / 293T cells transfected with an empty vector containing a Myc-His-tag (lower right) with ab323681 or 293T cells transfected with a Red fluorescent protein expression vector containing a Myc-His-tag (upper left) / 293T cells transfected with an empty vector containing a Myc-His-tag (lower left) with Rabbit IgG monoclonal [EPR25A] - Isotype Control (FITC) (ab223339). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 90% methanol for 30 min. Cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab323681) (1x 10⁶ in 100μl at 1 μg/ml (1/500)) for 30min at 22°C.

Isotype control antibody Rabbit IgG monoclonal [EPR25A] - Isotype Control (FITC) (ab223339) was used at the same concentration and conditions as the primary antibody. The cells were simultaneously stained with Myc.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable cells.