AB93579

FITC Anti-PD-1/CD279 抗体 [RMP1-30]

Name: FITC Anti-PD1 抗体 [RMP1-30] (ab93579) | Abcam
Brand: Abcam Limited
SKU: AB93579
Price: 45000 JPY
Availability: InStock

FITC Anti-PD-1/CD279 antibody [RMP1-30]

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(1 Publication)

Anti-PD-1/CD279 antibody [RMP1-30] conjugated to FITC - suitable for flow cytometry, and reacts with mouse samples. Clone [RMP1-30] is a rat monoclonal antibody and one of the most commonly used clones for flow cytometry.

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別名を表示する

CD279, Pd1, Pdcd1, Programmed cell death protein 1, Protein PD-1, mPD-1

1 Images

Flow Cytometry - FITC Anti-PD-1/CD279 antibody [RMP1-30] (AB93579)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-PD-1/CD279 antibody [RMP1-30] (AB93579)

Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 µg of FITC Rat IgG2b isotype control (open histogram), or 0.25 µg of ab93579 (colored histogram). Total viable cells were used for analysis.

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

RMP1-30

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Mouse

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing PD-1/CD279 protein. The exact immunogen used to generate this antibody is proprietary information.

Q02242

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "Use 0.5μg for 105-8 cells in 100μl.<a href='/products/primary-antibodies/fitc-rat-igg2b-kappa-monoclonal-eb149-10h5-isotype-control-ab136125'>ab136125</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody." } } }

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製品の詳細

ab93579 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.

Biological function summary

PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.

Pathways

PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.

PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 10485649, PubMed : 11209085, PubMed : 11698646, PubMed : 21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed : 11015443, PubMed : 11224527, PubMed : 18287011, PubMed : 18641123, PubMed : 22641383). Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation (PubMed : 22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2 : following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed : 11698646, PubMed : 22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).

See full target information PD-1/CD279

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 172:3994-8 PubMed15034010

2004

Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155).

Applications

Unspecified application

Species

Unspecified reactive species

Anja Fuchs,Marina Cella,Emanuele Giurisato,Andrey S Shaw,Marco Colonna

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-PD-1/CD279 抗体 [RMP1-30]

FITC Anti-PD-1/CD279 antibody [RMP1-30]

Flow Cytometry - FITC Anti-PD-1/CD279 antibody [RMP1-30] (AB93579)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise