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AB237382

FITC Anti-NCAM1 抗体 [EPR21827]

FITC Anti-NCAM1 antibody [EPR21827]

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(1 Publication)

Rabbit Recombinant Monoclonal NCAM1 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

別名を表示する

CD56, Ncam, Ncam1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

1 Images
Flow Cytometry - FITC Anti-NCAM1 antibody [EPR21827] (AB237382)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-NCAM1 antibody [EPR21827] (AB237382)

Flow cytometric analysis of unfixed Neuro-2 (right) and L-929 (left) cells with ab237382 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237382, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.

関連する標識済み抗体及び組成の異なる製品 (7)

  • Unconjugated

    Anti-NCAM1 antibody [EPR21827]

  • Carrier free

    Anti-NCAM1 antibody [EPR21827] - BSA and Azide free

  • 660 APC

    APC Anti-NCAM1 antibody [EPR21827]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-NCAM1 antibody [EPR21827]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-NCAM1 antibody [EPR21827]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-NCAM1 antibody [EPR21827]

  • 578 PE

    PE Anti-NCAM1 antibody [EPR21827]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21827

アイソタイプ

IgG

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Mouse, Rat

アプリケーション

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB119587

Human NCAM1 ELISA Kit (CD56)

Sandwich ELISA - Human NCAM1 ELISA Kit (CD56) (AB119587)

  • 5
  • 1
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

  • 0
  • 0
Primary Antibodies

AB307638

PE Anti-PDGFR alpha + PDGFR beta antibody [Y92] (C-terminal)

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-PDGFR alpha + PDGFR beta antibody [Y92] (C-terminal) (AB307638)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
See full target information Ncam1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 20:61 PubMed40399992

2025

Mechanism of LINC01018/miR-182-5p/Rab27B in the immune escape through PD-L1-mediated CD8 T cell suppression in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Su Hu,Guoshuo Chen,Aiping Luo,Hailin Zhao,Dan Li,Biao Peng,Jike Du,Dongdong Luo
View all publications
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