AB11729

FITC Anti-Myeloperoxidase 抗体 [2C7]

Name: FITC Anti-Myeloperoxidase 抗体 [2C7] (ab11729) | Abcam
Brand: Abcam Limited
SKU: AB11729
Price: 48000 JPY
Availability: InStock

FITC Anti-Myeloperoxidase antibody [2C7]

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(20 Publications)

Anti-Myeloperoxidase antibody [2C7] conjugated to FITC - suitable for intracellular flow cytometry, and reacts with human samples. Clone [2C7] is a mouse monoclonal antibody cited in 14 publications.

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別名を表示する

Myeloperoxidase, MPO

1 Images

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2C7] (AB11729)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2C7] (AB11729)

Staining of human peripheral blood granulocytes with ab11729 following permeabilisation with Leucoperm

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

2C7

アイソタイプ

IgG1

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt (Intra)

applications

免疫原

Recombinant Full Length Protein corresponding to Human MPO.

P05164

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1 - 1/10", "FlowCytIntra-species-notes": "<p>Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl. This antibody may be used for the flow cytometric detection of myeloid cells following membrane permeablisation. <a href='/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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製品の詳細

What is this antibody validated in?
FITC Anti-Myeloperoxidase antibody [2C7] (ab11729) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

Trusted by the scientific community
Anti-Myeloperoxidase [2C7] FITC (ab11729) was first used in a scientific publication in 2004 and has been cited over 10 times in peer-reviewed journals.

Related products
Antibody clone 2C7 is also available pre-conjugated to a variety of labels for your convenience – Anti-Myeloperoxidase FITC [2C7] (ab11729).

Other related products
We have a range of other formats of antibody clone [2C7] also available for your convenience: ab283263, Carrier free - ab283284, FITC - ab11729, PE - ab11730

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

Purified IgG prepared by affinity chromatography.

バッファー組成

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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文献 (20)

Recent publications for all applications. Explore the full list and refine your search

Kidney international reports 10:3070-3080 PubMed40980683

2025

Hemodiafiltration Attenuates NETosis Compared With High-Flux Hemodialysis in End-Stage Kidney Disease Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Lital Remez-Gabay,Olga Vdovich,Faten Y Andrawes Barbara,George Jiries,Etty Kruzel-Davila

Frontiers in immunology 16:1583587 PubMed40356921

2025

study: HIF-1α-dependent glycolysis enhances NETosis in hypoxic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Ye,Yanjun Wang,Qiying Xu,Juanli Liu,Ziqi Yang,Tana Wuren,Ri-Li Ge

NPJ breast cancer 11:5 PubMed39843922

2025

Triple-negative breast cancer modifies the systemic immune landscape and alters neutrophil functionality.

Applications

Unspecified application

Species

Unspecified reactive species

Noor A M Bakker,Hannah Garner,Ewald van Dyk,Elisa Champanhet,Chris Klaver,Maxime Duijst,Leonie Voorwerk,Iris Nederlof,Rosie Voorthuis,Marte C Liefaard,Marja Nieuwland,Iris de Rink,Onno B Bleijerveld,Hendrika M Oosterkamp,Lodewyk F A Wessels,Marleen Kok,Karin E de Visser

Frontiers in immunology 14:1265344 PubMed37841279

2023

High expression of P-selectin induces neutrophil extracellular traps via the PSGL-1/Syk/Ca/PAD4 pathway to exacerbate acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Xu,Ming Shi,Lu Ding,Yu Xia,Liang Luo,Xiaofang Lu,Xiaoying Zhang,David Y B Deng

Scientific reports 13:15633 PubMed37730741

2023

Secretomes of M1 and M2 macrophages decrease the release of neutrophil extracellular traps.

Applications

Unspecified application

Species

Unspecified reactive species

Aneta Manda-Handzlik,Adrianna Cieloch,Weronika Kuźmicka,Agnieszka Mroczek,Anna Stelmaszczyk-Emmel,Urszula Demkow,Małgorzata Wachowska

Immunology and cell biology 100:87-100 PubMed34714958

2021

Iron excess affects release of neutrophil extracellular traps and reactive oxygen species but does not influence other functions of neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Weronika Kuźmicka,Aneta Manda-Handzlik,Agnieszka Mroczek,Adrianna Cieloch,Aneta Moskalik,Urszula Demkow,Małgorzata Wachowska,Olga Ciepiela

Central-European journal of immunology 46:135-139 PubMed34764782

2021

Influence of iron- and zinc-chelating agents on neutrophil extracellular trap formation.

Applications

Unspecified application

Species

Unspecified reactive species

Weronika Kuźmicka,Aneta Moskalik,Aneta Manda-Handzlik,Urszula Demkow,Małgorzata Wachowska,Olga Ciepiela

Nutrients 13: PubMed33375275

2020

Zinc Supplementation Modulates NETs Release and Neutrophils' Degranulation.

Applications

Unspecified application

Species

Unspecified reactive species

Weronika Kuźmicka,Aneta Manda-Handzlik,Adrianna Cieloch,Agnieszka Mroczek,Urszula Demkow,Małgorzata Wachowska,Olga Ciepiela

International journal of molecular sciences 21: PubMed32707918

2020

Overexpression of Gene Makes Granulocyte-Like HL-60 Susceptible to Release Reactive Oxygen Species.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Mroczek,Adrianna Cieloch,Aneta Manda-Handzlik,Weronika Kuźmicka,Angelika Muchowicz,Małgorzata Wachowska

Journal of clinical medicine 9: PubMed32629885

2020

Blood Levels of Free-Circulating Mitochondrial DNA in Septic Shock and Postsurgical Systemic Inflammation and Its Influence on Coagulation: A Secondary Analysis of a Prospective Observational Study.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuel Schneck,Fabian Edinger,Matthias Hecker,Natascha Sommer,Oleg Pak,Norbert Weissmann,Andreas Hecker,Martin Reichert,Melanie Markmann,Michael Sander,Christian Koch

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Myeloperoxidase 抗体 [2C7]

FITC Anti-Myeloperoxidase antibody [2C7]

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2C7] (AB11729)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (20)

Abcam product promise