AB314952

FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control

Recombinant
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FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control Suitable for Flow Cyt (Intra) and reacts with samples.

1 Images

Flow Cytometry (Intracellular) - FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB314952)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB314952)

Flow cytometry overlay histogram showing HeLa cells stained with ab119688 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 90% methanol for 30 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab119688) (1x 10⁶ in 100μl at 1.0μg/ml) for 30min at 22°C.

Isotype control antibody (black line) was ab314952 (1.0μg/ml (1/500)) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

R312-MouseIgG1

アイソタイプ

IgG1

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

アプリケーション

Flow Cyt (Intra)

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }

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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Mouse IgG1 also known as immunoglobulin G1 is an important antibody isotype in mice. It has a molecular mass of approximately 150 kDa. Mouse IgG1 is found in the serum and extracellular fluids where it helps to mediate immune functions. This antibody is produced by plasma cells which originate from B lymphocytes. Its structure is comprised of two heavy chains and two light chains forming a Y-shaped molecule that facilitates antigen binding.

Biological function summary

Immunoglobulin G1 plays a significant role in adaptive immunity as it can engage with different cellular receptors and complement proteins to mediate immune responses. It does not function as part of a complex by itself but rather interacts with other immune system components to execute its tasks. Mouse IgG1 is known for its ability to neutralize pathogens agglutinate invading microbes and opsonize them for enhanced phagocytosis by immune cells like macrophages and neutrophils.

Pathways

The signaling processes associated with mouse IgG1 involve pathways such as the Fc gamma receptor pathway and the classical complement pathway. Engagement of its Fc region with Fc gamma receptors on the surface of immune cells leads to effector functions like phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). In these pathways proteins like complement component C1q and other Fc gamma receptor subtypes play key roles in modulating the immune responses initiated by IgG1.

Immunoglobulin G1 is relevant in conditions like autoimmune diseases and allergic reactions where inappropriate immune activation or regulation can be observed. In autoimmune diseases for example IgG1 can form immune complexes that deposit in tissues and activate inflammatory cascades involving proteins like complement factors and other immunoglobulin subclasses. Its involvement in allergic responses often involves IgG1 competing with IgE antibodies for antigen binding altering the allergic response dynamics.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control