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FITC Mouse IgG - Isotype Control (ab37356)

Q&A (3)References (4)

製品の概要

  • 製品名

    FITC Mouse IgG - Isotype Control
    Mouse アイソタイプ・コントロール 製品一覧

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • 特異性

    This antibody has been selected as an isotype control as it has no known specificity.

  • アプリケーション

    適用あり: ELISA, Flow Cytmore details

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • バッファー

    pH: 7.4
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

  • 別名

    • Mouse Isotype Control

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab37356の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent dilution.
Flow Cyt
Use 1µg for 106 cells.
特記事項
ELISA
Use at an assay dependent dilution.
Flow Cyt
Use 1µg for 106 cells.

参考文献 (4)

ab37356 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab37356 は 4 報の論文で使用されています。

  • Strekalova E  et al. Preclinical Breast Cancer Models to Investigate Metabolic Priming by Methionine Restriction. Methods Mol Biol 1866:61-73 (2019). PubMed: 30725408
  • Hu C  et al. Identification of oxidative stress-induced gene expression profiles in cavernosal endothelial cells. Mol Med Rep 11:2781-8 (2015). PubMed: 25523828
  • Barone A  et al. Sialyl-lactotetra, a novel cell surface marker of undifferentiated human pluripotent stem cells. J Biol Chem 289:18846-59 (2014). Flow Cyt . PubMed: 24841197
  • Laughlin ST & Bertozzi CR Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation. Nat Protoc 2:2930-44 (2007). PubMed: 18007630

レビューと Q&A

Q&A
質問を送る

1-3 of 3 Q&A

Question

A customer wishes to get an isotype control for the above antibody and I suggested:
AB37356 Abcam Mouse IgG (FITC) - Isotype Control (100 ug)
However, the customer thinks it might not be suitable and ab18435 Mouse IgG1, kappa monoclonal [MOPC-21] (FITC) - Isotype control
might be a better choice?
Please kindly advise on how we may proceed.
I look forward to your reply!

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Answer



The anti-CD4 antibody [RPA-T4] (FITC), ab86886, is a Mouse IgG1. The best isotype control for ab86886 would be a Mouse IgG1 FITC conjugated such as ab18435.

ab37356 would be a good isotype control for a Mouse polyclonal IgG FITC conjugated.

Read More

Question

I can't find a mouse polyclonal control on your website. Could you please tell me if you do sell one or suggest an alternative for fluorescein conjugation and flowcytometry.

Read More
Answer

Thank you for contacting us.

We have the following mouse poyclonal antibody to be used as an isotype control in flow cytometry:

ab37355 (unconjugated; https://www.abcam.com/Mouse-IgG-ab37355.html)

ab37356 (FITC conjugated; https://www.abcam.com/Mouse-IgG-FITC-Isotype-Control-ab37356.html)

In case of the unconjugated isotype control, a conjugation kit can be used to label the antibody: https://www.abcam.com/Easylink
We do carry several FITC conjugation kits: ab102885, ab102884 and ab102883.

I hope this information is helpful to you. Please let me know if you need any more advice or information.

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Question

この製品のisotype controlを教えてください。

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Answer

いつもお世話になっております。 この度はお問合せいただきましてありがとうございます。 お客様が探しているisotype controlはab37356になります。是非添付しているデータシートのリンクでご確認お願いいたします。

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