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FITC Anti-MHC class II I E kappa 抗体 [14-4-4S] (ab25023)

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Flow Cytometry - FITC Anti-MHC class II I E kappa antibody [14-4-4S] (ab25023)

    Key features and details

    • FITC Mouse monoclonal [14-4-4S] to MHC class II I E kappa
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    Biotin PE Unconjugated

    製品の概要

    • 製品名

      FITC Anti-MHC class II I E kappa antibody [14-4-4S]
      MHC class II I E kappa 一次抗体 製品一覧

    • 製品の詳細

      FITC Mouse monoclonal [14-4-4S] to MHC class II I E kappa

    • 由来種

      Mouse

    • 標識

      FITC. Ex: 493nm, Em: 528nm

    • 特異性

      ab25023 recognises MHC class II I E kappa alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. It cross reacts with alloantigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express this antigen.

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Mouse

    • 免疫原

      Tissue, cells or virus corresponding to Mouse MHC class II I E kappa. C3H mouse skin graft and splenocytes

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • バッファー

      pH: 7.4
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 精製度

      Affinity purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      14-4-4S

    • アイソタイプ

      IgG2a

    • 軽鎖の種類

      kappa

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab25023の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    特記事項
    追加情報
    BL: Use at an assay dependent dilution for in vitro blocking of antigen presentation.
    Flow Cyt: Use 1µg for 106 cells.
    FuncS: Use at an assay dependent dilution for complement dependent cytotoxicity studies and in vitro induction of antigen specific B cell differentiation.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    ターゲット情報

    • 関連性

      A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.

    • 細胞内局在

      Type I membrane protein

    • 別名

      • H2 Ea antibody
      • H2-Ea antibody

    画像

    • Flow Cytometry - FITC Anti-MHC class II I E kappa antibody [14-4-4S] (ab25023)
      Flow Cytometry - FITC Anti-MHC class II I E kappa antibody [14-4-4S] (ab25023)

      Flow cytometry analysis staining MHC class II I E kappa in mouse splenocytes using ab25023 at a dilution of 1 ug/106 cells.

    参考文献 (1)

    ab25023 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab25023 は 1 報の論文で使用されています。

    • Guan J  et al. Enhanced immunocompatibility of ligand-targeted liposomes by attenuating natural IgM absorption. Nat Commun 9:2982 (2018). PubMed: 30061672

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