FITC Anti-Ly6g 抗体 [RB6-8C5] (ab25024)
Key features and details
- FITC Rat monoclonal [RB6-8C5] to Ly6g
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
-
製品名
FITC Anti-Ly6g antibody [RB6-8C5]
Ly6g 一次抗体 製品一覧 -
製品の詳細
FITC Rat monoclonal [RB6-8C5] to Ly6g -
由来種
Rat -
標識
FITC. Ex: 493nm, Em: 528nm -
特異性
ab25024 recognises mouse Mouse Ly-6G. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Ly6g. Mouse granulocytes
-
ポジティブ・コントロール
- Rat IgG2b (FITC) isotype control
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
-
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
RB6-8C5 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
- redFluor™ 710 Anti-Ly6g antibody [RB6-8C5] (ab242065)
- PE Anti-Ly6g antibody [RB6-8C5] (ab24884)
- APC Anti-Ly6g antibody [RB6-8C5] (ab25273)
- violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)
- Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377)
- PE/Cy7® Anti-Ly6g antibody [RB6-8C5] (ab25514)
- Biotin Anti-Ly6g antibody [RB6-8C5] (ab25680)
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25024の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt | (5) |
1/100.
ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
---|
Flow Cyt
1/100. ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
関連性
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells. -
細胞内局在
Attached to the membrane by a GPI-anchor. -
参照データベース
- Entrez Gene: 546644 Mouse
- SwissProt: P35461 Mouse
-
別名
- Gr-1 antibody
- Gr1 antibody
- Ly-6G.1 antibody
see all
画像
-
Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 0.5µg (right panel), Rat IgG2b (FITC) isotype control at 0.5µg (left panel).
-
BALB/c bone marrow cells were stained with either rat IgG2b-FITC (as an isotype control) or rat anti-mouse Ly-6G-FITC (ab25024) at a concentration of 1ug/106 cells. Large cells were then gated and analyzed on a flow cytometer.
-
Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 at 0.5µg.
-
Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)Image courtesy of an anonymous Abreview.
ab25024 at a 1/100 dilution detecting Ly6g (Gr1) in murine blood cells by Flow Cytometry. Gating strategy was for monocytes.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (19)
ab25024 は 19 報の論文で使用されています。
- Fan Z et al. Sympathetic Nerve-Mediated Fellow Eye Pain During Sequential Cataract Surgery by Regulating Granulocyte Colony Stimulating Factor CSF3. Front Cell Neurosci 16:841733 (2022). PubMed: 35281296
- Tian S et al. Curcumin inhibits the growth of liver cancer by impairing myeloid-derived suppressor cells in murine tumor tissues. Oncol Lett 21:286 (2021). PubMed: 33732362
- Nugraha B et al. The p38/MK2 Axis in Monocytes of Fibromyalgia Syndrome Patients: An Explorative Study. Medicina (Kaunas) 57:N/A (2021). PubMed: 33921654
- Lv D et al. A novel cell-based assay for dynamically detecting neutrophil extracellular traps-induced lung epithelial injuries. Exp Cell Res 394:112101 (2020). PubMed: 32474064
- Ding F et al. microRNA-375 released from extracellular vesicles of bone marrow mesenchymal stem cells exerts anti-oncogenic effects against cervical cancer. Stem Cell Res Ther 11:455 (2020). PubMed: 33109266