FITC Anti-LOX 1 抗体 [23C11] (ab81710)
![Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)](https://www.abcam.co.jp/ps/products/81/ab81710/Images/ab81710-454449-fitc-anti-lox1-antibody-23c11-flow-cyt-human-pbmc.JPG "Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)")
Key features and details
- FITC Mouse monoclonal [23C11] to LOX 1
- Suitable for: ICC/IF, Flow Cyt, Functional Studies
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-LOX 1 antibody [23C11]
LOX 1 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [23C11] to LOX 1 -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: ICC/IF, Flow Cyt, Functional Studiesmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Recombinant fragment within Human LOX 1 aa 50-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- Human APCs
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特記事項
ab81710 neutralizes the biological activity of LOX 1. It also inhibits Hsp70 binding to dendritic cells and Hsp70 induced antigen cross representation. In vivo, targeting LOX1 with a tumour antigen using anti LOX 1 antibody ab81710 induces anti tumour immunity.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
特記事項（精製）
Purified, 0.2 µm filtered solution. -
一次抗体 備考
ab81710 neutralizes the biological activity of LOX 1. It also inhibits Hsp70 binding to dendritic cells and Hsp70 induced antigen cross representation. In vivo, targeting LOX1 with a tumour antigen using anti LOX 1 antibody ab81710 induces anti tumour immunity. -
ポリ/モノ
モノクローナル -
クローン名
23C11 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 5µl for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| Functional Studies |
Use at an assay dependent concentration.
Antibody totally prevented Hsp70 binding to LOX-1-CHO but not mock-transfected CHO cells. |
| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use 5µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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Functional Studies
Use at an assay dependent concentration. Antibody totally prevented Hsp70 binding to LOX-1-CHO but not mock-transfected CHO cells. |
ターゲット情報
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機能
Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. -
組織特異性
Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. -
関連疾患
Note=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. -
配列類似性
Contains 1 C-type lectin domain. -
ドメイン
The cytoplasmic region is required for subcellular sorting on the cell surface.
The C-type lectin domain mediates the recognition and binding of oxLDL. -
翻訳後修飾
The intrachain disulfide-bonds prevent N-glycosylation at some sites.
N-glycosylated. -
細胞内局在
Cell membrane. Secreted. A secreted form also exists. - Information by UniProt
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参照データベース
- Entrez Gene: 4973 Human
- Entrez Gene: 108078 Mouse
- Omim: 602601 Human
- SwissProt: P78380 Human
- SwissProt: Q9EQ09 Mouse
- Unigene: 412484 Human
- Unigene: 293626 Mouse
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別名
- C-type lectin domain family 8 member A antibody
- CLEC8A antibody
- hLOX 1 antibody
see all
画像
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Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)
Flow cytometric analysis showing expression of LOX1 on human PBMC cells at 10 µg/mL concentration.
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![Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)](https://www.abcam.co.jp/ps/products/81/ab81710/Images/LOX-1-Primary-antibodies-ab81710-1.jpg)
Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)Overlay histogram showing A549 cells stained with ab81710 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81710, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 FITC (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab81710 は 4 報の論文で使用されています。
- Zhang P et al. Expression of OLR1 gene on tumor-associated macrophages of head and neck squamous cell carcinoma, and its correlation with clinical outcome. Oncoimmunology 12:2203073 (2023). PubMed: 37089448
- Barth H et al. Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells. J Virol 82:3466-79 (2008). PubMed: 18216094
- Jeannin P et al. Complexity and complementarity of outer membrane protein A recognition by cellular and humoral innate immunity receptors. Immunity 22:551-60 (2005). ICC/IF, Flow Cyt ; Human . PubMed: 15894273
- Delneste Y et al. Involvement of LOX-1 in dendritic cell-mediated antigen cross-presentation. Immunity 17:353-62 (2002). Human . PubMed: 12354387