FITC Anti-Lambda Light chain 抗体 (ab4211)
Key features and details
- FITC Rabbit polyclonal to Lambda Light chain
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
製品の概要
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製品名
FITC Anti-Lambda Light chain antibody
Lambda Light chain 一次抗体 製品一覧 -
製品の詳細
FITC Rabbit polyclonal to Lambda Light chain -
由来種
Rabbit -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human Lambda Light chain. Pool of human Lambda light chain isolated from the urine of patients with Bence Jones Proteinuria.
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ポジティブ･コントロール
- IHC-P: Human tonsil tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
精製度
IgG fraction -
特記事項（精製）
Traces of contaminating antibodies have been removed by solid phase absorption. Only the lambda precipitation line appeared in cross immunoelectrophoresis. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4211の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/20 - 1/40.
Pretreat tissue with Proteinase K for 30 minutes at 37°C. Incubate with primary antibody for 30-60 minutes at room temperature. |
| 特記事項 |
|---|
|
IHC-P
1/20 - 1/40. Pretreat tissue with Proteinase K for 30 minutes at 37°C. Incubate with primary antibody for 30-60 minutes at room temperature. |
ターゲット情報
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関連性
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 3535 Human
- Entrez Gene: 3546 Human
- Entrez Gene: 8217 Human
- Omim: 147220 Human
- SwissProt: P01701 Human
- SwissProt: P01842 Human
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別名
- Bence Jones protein antibody
- BJP antibody
- Constant region of lambda light chains antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Lambda Light chain antibody (ab4211)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue labeling Lambda Light chain with ab4211.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab4211 は 1 報の論文で使用されています。
- Kang SU et al. DIM-C-pPhtBu induces lysosomal dysfunction and unfolded protein response - mediated cell death via excessive mitophagy. Cancer Lett 504:23-36 (2021). PubMed: 33556544