AB77275

FITC Anti-Lambda Light chain 抗体 [4C2]

Name: FITC Anti-Lambda Light chain 抗体 [4C2] (ab77275) | Abcam
Brand: Abcam Limited
SKU: AB77275
Price: 76000 JPY
Availability: InStock

FITC Anti-Lambda Light chain antibody [4C2]

Be the first to review this product! Submit a review

(1 Publication)

Anti-Lambda Light chain antibody [4C2] conjugated to FITC - suitable for flow cytometry, and reacts with human samples. Clone [4C2] is a mouse monoclonal antibody.

Design your panel with our interactive flow cytometry panel builder. Click here.

別名を表示する

Immunoglobulin lambda variable 1-51, Ig lambda chain V-I region BL2, Ig lambda chain V-I region EPS, Ig lambda chain V-I region NEW, Ig lambda chain V-I region NIG-64, IGLV1-51

3 Images

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human Ig lambda light chain (4C2) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig lambda light chain (4C2) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Separation of human Ig lambda light chain positive B-lymphocytes (red-filled) from Ig lambda light chain negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig lambda light chain (4C2) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

4C2

アイソタイプ

IgG1

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt

applications

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." }, "Goat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." }, "Guinea pig": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." }, "Hamster": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." }, "Rabbit": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "21 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." }, "Sheep": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Size-exclusion chromatography

精製に関する特記事項

ab77275 is conjugated with Fluorescein isothiocyanate (FITC) under optimumconditions. The reagent is free of unconjugated FITC and adjusted for direct use.

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Lambda Light Chain also known as lambda chain or lambda protein forms a part of immunoglobulins (antibodies). Its mass typically ranges between 22 to 24 kDa. This protein is expressed primarily in B cells. Specifically it pairs with heavy chains to form the complete antibody molecule. It participates in the antigen recognition process supporting the immune system's defense mechanisms.

Biological function summary

Lambda Light Chain participates in immunological functions by contributing to the antigen-binding site of antibodies. It exists as a part of the immunoglobulin complex. The lambda chain alongside heavy chains forms the variable part of antibodies allowing specificity in antigen recognition. Its variation leads to a diverse antibody repertoire which is important for recognizing a wide range of pathogens.

Pathways

Lambda Light Chain fits into pathways involving adaptive immune responses. It is important in antigen processing and presentation pathways. In this context it interacts with the heavy chains which together drive the specificity and efficiency of immune response pathways. It also associates with proteins like the major histocompatibility complex (MHC) which facilitates antigen presentation to T cells.

Lambda Light Chain associates with multiple myeloma a cancer of plasma cells and light chain amyloidosis a disorder where light chains accumulate as amyloid fibrils in tissues. In these conditions proteins like immunoglobulin heavy chain and albumin often interact with or are affected by lambda chains providing insights into disease mechanisms and potential targets for therapeutic intervention.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed : 24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGLV1-51

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular neuroscience : MN 74:98 PubMed39414746

2024

Effects of Electroacupuncture at Varied Frequencies on Analgesia and Mechanisms in Sciatic Nerve Cuffing-Induced Neuropathic Pain Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kexin Fang,Wen Cheng,Bin Yu

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Lambda Light chain 抗体 [4C2]

FITC Anti-Lambda Light chain antibody [4C2]

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise