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FITC Anti-IgE 抗体 [BE5] (ab49434)

Submit a review Q&A (1)References (1)

Key features and details

  • FITC Mouse monoclonal [BE5] to IgE
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

製品の概要

  • 製品名

    FITC Anti-IgE antibody [BE5]
    IgE 一次抗体 製品一覧

  • 製品の詳細

    FITC Mouse monoclonal [BE5] to IgE

  • 由来種

    Mouse

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length native protein (purified) corresponding to Human IgE.

  • 特記事項

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...

  • 精製度

    Size exclusion

  • ポリ/モノ

    モノクローナル

  • クローン名

    BE5

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab49434の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
Flow Cyt
Use at an assay dependent concentration.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性

    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.

  • 細胞内局在

    Cell Membrane

  • 参照データベース

    • 別名

      • Igh2 antibody
      • IGHE antibody
      • IGHEP1 antibody
      • Immunoglobulin heavy constant epsilon antibody

    参考文献 (1)

    ab49434 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab49434 は 1 報の論文で使用されています。

    • Gervais FG  et al. Pharmacological characterization of MK-7246, a potent and selective CRTH2 (chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells) antagonist. Mol Pharmacol 79:69-76 (2011). Flow Cyt ; Human . PubMed: 20943773

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Hi I was wondering whether you could tell me which epitope this antibody binds to? We are conducting some experiments whereby we are crosslinking the IgE-receptor and then looking at expression but need to know that the epitope recognised by your antibody is not the same one as the antibody that we are using t stimulate our cells. Many thanks in advance for your assistance

    Read More
    Answer

    Unfortunately, having consulted with the source of this antibody, the epitope of the antibody is not known. However, it has been established that the antibody detects a different epitope to that of clones 4G7 and 4H10 (4G7: ab774 and 4H10: ab774 and ab77121 PE-dynamics conjugated). If you find there is an overlap with ab49434 and your other antibody you could change to using one of these alternatives. Otherwise we have a few anti-human IgE of which the epitope is know. ab7294 and ab7295 are specific for the Ce3 and Ce2 region respectively. ab7378 is specific for the Fc region and ab7382 is specific for the epsilon chain. I hope this information has been of help to you. If you have any further questions please do not hesitate to ask.

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