FITC Anti-Human Serum Albumin 抗体 (ab63498)

Thank you for your reply. Indeed, the approach of competing off the labelled antibody might also be also a suitable control, although it would not take into account any unspecific binding of the antibody. I am sorry that we do not have at least a suitable, ready-to-use isotype control available. We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. From my personal experience the FMO control would be the most appropriate control. It is also being requested more and more for publishing. I hope this information is nevertheless helpful to you and wish you the best of luck with your research. Should you have any further questions, please do not hesitate to contact us.

Thank you for contacting us. Unfortunately, I would not recommend using a chicken antibody against a different target (such as ab1394 or ab63504), as it cannot be guaranteed that they do not react with any human antigen. I would rather suggest to use a chicken IgY polyclonal isotype control, as these isotype controls are specifically tested not to cross react. As such we currently have ab37382 available (Click here (or use the following: https://www.abcam.com/Chicken-IgY-polyclonal-Isotype-Control-BSA-and-Azide-free-ab37382.html).). As this antibody however is not labelled with FITC, I may recommend our EasyLink Conjugation kit (ab102883 - ab102885, see below and attached). For the compensation and set-up of the flow cytometer, I would suggest to use ab63498 to ensure the optimal settings. EasyLink antibody conjugation kits: https://www.abcam.com/easylink Click here (or use the following: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-1-x-1mg-FITC-ab102883.html). Having said this however, I may let you know that isotype controls do not really represent a good negative control. The reasons become apparent in your particular case: The control antibody is of a different preparation ('chicken', or in case of monoclonals a different clone) and therefore may show varying binding properties compared to the specific antibody of interest (ab63498). Furthermore, as the conjugation conditions may have been different, the labelling with the fluorochrome can vary which can be a very important issue in flow cytometry. As a result of different antibody-to-fluorochrome ratios and/or different fluorochrome batches, the control antibody might indeed show a very different staining pattern, and even if it does not bind at all (as it is supposed to) can for these reasons not directly be compared to the antibody of interest. Instead, in flow cytometry another type of control is often used to determine positivity for the marker of interest. This control is termed fluorescence minus one or FMO and works like an isotype control without the actual antibody. FMO controls are set up by leaving out one of the antibodies in your staining panel. You may find more information about this type of control in the latest publications or on flow cytometry websites, such as http://www.flowcytometryuk.org/, http://www.cyto.purdue.edu/ or http://www.isac-net.org . Plus, your local flow cytometry community (core facility?) might be happy to help and advise in the use of optimal controls which are required for publishing the results. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.